己烯雌酚对大鼠腹腔内睾丸下降的影响及相关因子的变化

目的 观察不同剂量己烯雌酚(DES)致大鼠腹腔内隐睾及其对睾丸胰岛素样因子3(INSL3)、睾丸类固醇生成因子-1(SF-1)、引带同源盒基因-A10(HOXA10)表达的影响.方法 50只SD孕鼠随机分为5组,每组10只,在孕期第13.5天(E 13.5)分别皮下注射二甲基亚砜(对照组)和DES2.5、5、10、20 mg/kg,在E 19.5取出胚胎雄鼠,体视显微镜下观察后计算死胎率、睾丸下降程度;RT-PCR检测INSL3、SF-1及HOXA10 mRNA水平,Western blot检测INSL3蛋白水平.结果 ①与较对照组比较,DES处理各组的死胎率明显增高、睾丸下降程度明显较差(P＜0.01),且均呈剂量效应关系;②DES显著下调了INSL3、SF-1 mRNA和INSL3蛋白的表达,且呈剂量效应关系(P＜0.01);③2.5 mg/kg的DES未影响HOXA10的表达(P＞0.05),5、10、20 mg/kg的DES均下调HOXA10表达(P＜0.05).结论 DES通过下调大鼠睾丸INSL3表达致腹腔内睾丸下降受阻,且呈剂量效应关系.DES通过剂量效应下调SF-1来下调INSL3的表达.低剂量DES(2.5 mg/kg)未影响引带HOXA10表达,HOXA10在高剂量(5、10、20 mg/kg)DES诱导的大鼠腹腔内隐睾中发挥了作用.

Effect of diaethyistilbestrol on the transabdomial phase of testicular descent in the rat

Objective To study the effect of diaethylstilbestrol on the transabdomial phase of testicular descent in the rat.Methods Fifty E 13.5(embryonic day 13.5)pregnant female SD rats were randomlv divided into five groups that received a subcutaneous injection of DMSO,2.5、5、10、20 mg/kg DES respectively.Male offspring were killed at E 19.5,and then fetal mortality,the degree of transabdominal testicular ascent(DTA)was determined by a stereomicroscope.The tuRNA expressions of INSL3、SF-1 in the testis and HOXA10 in the gubernaculums were determined by RT-PCR.The expression of INSL3 protein was determined by western blot.Results Higher fetal mortality,larger DTA were induced by DES dose-dependently(P＜0.01).DES down-regulated the expression of INSL-3 and SF-1 in a dose-effect manner(P＜0.01).Compared with the DMSO group,the expression of HOXA10 mRNA in the group of 2.5 mg/kg was not significant difference(P＞0.05),the expression of HOXA10 mRNA in other groups were down-regulated statistically(P＜0.05).Conclusions DES inhibited transabdominal testicular descent dose-dependently via dose-dependent down-regulating the expression of INSL3,which was induced by dose-dependent down-regulating the expression of SF1.HOXA10 may play no role in low-dosage DES induced abdominal cryptorchidism,but down-regulated HOXA10 mRNA was involved in high-dosage DES induced ones.