内皮素与糖尿病造影剂肾病发生的关系研究

目的 :探讨内皮素 (ET)系统与糖尿病 (DM )造影剂肾病发生的关系。方法 :建立单侧肾切除糖尿病肾病模型 ,观察静脉注入 76 %泛影葡胺 (10mg/kg)后血肌酐、肌酐清除率和血浆ET - 1的变化情况 ,以及肾脏ET- 1、ET受体A、B(ETR -A、ETR -B)的表达情况。结果 :单侧肾切除糖尿病大鼠喂养 8周时 ,血肌酐较正常大鼠升高 ,肌酐清除率、血浆内皮素 - 1浓度较正常大鼠明显降低 ,有显著性差异 (P <0 .0 5 ) ;糖尿病大鼠禁水2 4h后注射 76 %泛影葡胺 ,血肌酐升高 ,内生肌酐清除率下降 ,与糖尿病对照组有显著性差异 (P <0 .0 5 ) ,血浆ET - 1浓度较糖尿病对照组有所升高 ,但无显著性差异。DM对照组肾小管间质ET - 1、ETR -A、ETR -B表达较正常对照组显著增加 ,注射 76 %泛影胺后表达增加更明显。结论 :糖尿病肾功能不全时 ,造影剂肾病的发生与内皮素系统特别是肾脏局部内皮素系统可能有关。

ROLE OF ENDOTHELIN IN RADIOCONTRAST ACUTE RENAL FAILURE IN DIABETIC RATS

Objective:To determine the role of endothelin in radiocontrast actue renal failure in diabetic rats.Methods:Eighteen rats were randomly assigned into 3 groups:normal cotrols, streptotcin diabetic rats,streptotocin diabetic rats injected diatrizoate (10ml/kg).Renal function was measured before and after injection,and plasma endothelin-1 was also measured.The protein expression of ET-1,ETR-A,ETR-B was determined by immuno-histochemistry at 8th week.Results:In the diabetic rats,diatrizoate reduced the glomerular filtration rate (GFR).The plasma ET-1 concentration in diabetic rats was lower than normal rats,and diatrizoate has little effect on the plasma ET-1 concentration.Immunochemistry analysis showed over-expression of ET-1,ETR-A,ETR-B.Conclusion:There is a close relation between renal local endothelin and acute renal failure in diabetic rats induced by contrast media.