骨髓间充质干细胞体外定向诱导内皮祖细胞的实验研究

目的:研究兔骨髓间充质干细胞(mensenchymal stem cell,MSC)在体外定向诱导分化为内皮祖细胞(endothelial progenitor cell,EPC)的状况。方法:取兔髂骨骨髓,经密度梯度离心法分离并培养骨髓间充质干细胞,实验组细胞在内皮细胞生长添加剂、血管内皮生长因子、成纤维细胞生长因子等诱导条件下定向分化为内皮祖细胞。对照组不干预,继续培养。结果:实验组细胞集落接近融合时形态呈现“鹅卵石”样或“铺路石”样外观,流式细胞仪检测发现实验组细胞较对照组高度表达CD133、CD34(P<0．05)。结论:体外诱导可使骨髓间充质干细胞分化为内皮祖细胞,并有效扩增。

Experimental study on the differentiation of mesenchymal stem cells of bone marrow to endothelial progenitor cell in vitro

Objective:To study the differentiation of mesenchymal stem cells(MSCs) from rabbit bone marrow in vitro. Methods: Isolated MSCs from rabbit bone marrow were cultured in ceil culture medium with endothelial cell growth supplements (ECGS),vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in experiment group and in other group it wasn't induced by growth factors. MSCs were identified by means of phase-contrast microscope and flowcytometer. Results: The experiment group cell form coalseced to monolayer,which shows a cobblestone sample when observed with phase-contrast microscope. The expression of CD133,Cd34 in experiment group was also significantly increased compared with control cell(P<0. 05). Conclusion: After induced by ECGS and growth factor, mesenchymal stem cells can different into endothelial progenitor cell in vitro.