三阴性乳腺癌(TNBC)组织中CCR4和CCR7的表达及意义

 目的 探讨三阴性乳腺癌(triple negative breast cancer,TNBC)组织中CC族趋化因子受体4 (cc chemokine receptor 4,CCR4)和CCR7的表达意义及其与临床病理特征和预后之间的相关性。方法 应用免疫组织化学染色SP法检测TNBC、HER 2过表达型乳腺癌、TNBC癌旁组织及乳腺良性肿瘤(3组,各38例)和管腔型乳腺癌组织(40例)中CCR4和CCR7的表达。结果 在TNBC组织中,CCR4和CCR7的表达率分别为47.4%(18/38)和50%(19/38),与非TNBC组相比,差异有统计学意义(P值分别为0.001和0.004)。CCR4表达与TNBC肿瘤直径(P=0.023)、腋窝淋巴结转移(P=0.006)、肿瘤分期(P=0.016)和远处转移(P=0.028)相关。Kaplan Meier生存分析显示CCR4表达与TNBC患者总生存率(overall survival,OS)相关(P=0.018)。CCR7表达和TNBC患者的腋窝淋巴结转移相关(P=0.038),而与TNBC患者无病生存率(disease free survival,DFS)和OS无关。多因素分析显示腋窝淋巴结转移是TNBC患者DFS的独立预测因素(P=0.025),而CCR4是TNBC患者OS的独立预测因素(P=0.022)。结论 TNBC中CCR4和CCR7的表达与腋窝淋巴结转移相关;CCR4可作为TNBC患者预后的独立预测指标。

Expression and significance of CCR4 and CCR7 in triple-negative breast cancer (TNBC)

Objective To investigate the relationship between the expression of both CC chemokine receptor 4 (CCR4) and CCR7 and clinical pathology as well as prognosis in triple negative breast cancer (TNBC).Methods The expression of CCR4 and CCR7 in TNBC,Her 2 over expression breast cancer,TNBC paracancerous tissues and mammary gland benign tumor (38 cases each for the 3 groups),and 40 cases of lumina breast cancer were assessed by immunohistochemical technology SP method.Results The positive rate of CCR4 and CCR7 in TNBC were 47.4% (18/38) and 50% (19/38), respectively.The difference between the expression of CCR4 and CCR7 in TNBC and that in non TNBC showed a statistical significance (P=0.001 and 0.004,respectively).The expression of CCR4 in TNBC was correlated with tumor size (P=0.023),axilla lymph node status(P=0.006),tumor stage (P=0.016) and distance transfer (P=0.028).CCR4 expression showed by Kaplan Meier survival analysis was correlative withoverall survival (OS) (P=0.018) time of TNBC patients.The expression of CCR7 was correlative with axilla lymph node status (P=0.038),but was not correlative with OS and disease free survival time (DFS) of TNBC patients.Multivariate analysis showed that axilla lymph node status was a significant independent prognostic factor for DFS (P=0.025),and CCR4 expression was a significant independent prognostic factor for OS (P=0.022).Conclusions The expressions of CCR4 and CCR7 in TNBC were correlative with axilla lymph node status,and CCR4 expression was an independent predictor of TNBC.