| SARS冠状病毒S蛋白S1片段基因克隆和变异分析 |
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| 引用本文： | 吴淑珍,张洪勤,包其郁,毕云天,黄慧聪. SARS冠状病毒S蛋白S1片段基因克隆和变异分析[J]. 温州医学院学报, 2005, 35(1): 16-18 |
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| 作者姓名： | 吴淑珍 张洪勤 包其郁 毕云天 黄慧聪 |
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| 作者单位： | 1. 温州医学院,分子生物学研究中心,浙江,温州,325027
2. 温州医学院,寄生虫学教研室,浙江,温州,325027 |
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| 基金项目： | 浙江省温州市科技局资助项目 |
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| 摘 要： | 目的:获得SARS冠状病毒S蛋白S1片段基因的克隆.方法:将SARS病毒RNA基因组逆转录合成cDNA,PCR得到阳性条带,将其亚克隆到pUCm-T载体中进行酶切及测序分析,并与Genbank中核苷酸序列进行同源性分析.结果:阳性克隆经酶切鉴定,目的基因片段长度为1905 bp,与Genbank中另外45株的SARS病毒株的S蛋白S1片段基因比较,共有13个位点发生突变,其中有8处为同义突变,5处为错义突变.结论:成功克隆SARS冠状病毒S蛋白S1片段基因,为该基因的表达及功能研究奠定了基础.
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| 关 键 词： | SARS冠状病毒 S蛋白S1片段 基因克隆 |
| 文章编号： | 1000-2138(2005)01-0016-03 |
| 修稿时间： | 2004-03-11 |
Sequencing and variation analyzing of the cloned S1 fragment of spike glycoprotein gene of the SARS coronavirus |
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| WU Shu-zhen,ZHANG Hong-qin,BAO Qi-yu,et al.. Sequencing and variation analyzing of the cloned S1 fragment of spike glycoprotein gene of the SARS coronavirus[J]. Journal of Wenzhou Medical College, 2005, 35(1): 16-18 |
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| Authors: | WU Shu-zhen ZHANG Hong-qin BAO Qi-yu et al. |
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| Affiliation: | WU Shu-zhen,ZHANG Hong-qin,BAO Qi-yu,et al. Molecular Biology Research Centre of Wenzhou Medical College,Wenzhou 325027 |
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| Abstract: | Objective:To sequence and analyze the cloned S1 fragment of spike glycoprotein gene of the SARS coronavirus.Methods:The S1 fragment of spike glycoprotein gene was obtained by RT-PCR from the SARS coronavirus genome. PCR product was ligated into pUCm-T vector and sequenced.Results:The cloned segment was 1905 bp in length. Compared with the other 45 SARS genomes available in Genbank database, 13 nucleotide variations were identified, of which 8 loci belonged to same sense mutation and 5 loci belonged to missense mutation.Conclusion:The S1 fragment of spike glycoprotein gene of the SARS coronavirus is cloned using RT-PCR,which can be used for expression and function research. |
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| Keywords: | SARS coronavirus S1 fragment of spike glycoprotein gene gene cloning |
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