戈雷拉肽对单侧输尿管梗阻大鼠模型肾间质纤维化的保护作用

目的观察戈雷拉肽对肾间质纤维化（RIF）的保护作用。方法15只健康雄性SD大鼠随机分为假手术组（Ⅰ组）、单侧输尿管梗阻（UUO）模型组（Ⅱ组）及戈雷拉肽治疗组（Ⅲ组）。术后第15d摘取梗阻侧肾脏，通过Masson染色观察肾间质炎症细胞的数量及纤维化程度；免疫组织化学方法观察转化生长因子（TGF-β1）及结缔组织生长因子（CTGF）表达情况。结果模型组24h尿蛋白定量、肌酐、尿素氮高于假手术组及戈雷拉肽治疗组（P〈0．01）；戈雷拉肽治疗组24h尿蛋白定量、肌酐、尿素氮高于假手术组（P〉0．05）。与假手术组相比，模型组肾间质炎症细胞数量增多、RIF程度明显、TGF-β1、CTGF表达增强（P〈0．01），戈雷拉肽治疗组与模型组相比，以上各项指标明显降低（P〈0．01），高于假手术组（P〈0．05）。结论戈雷拉肽通过下调TGF-β1、CTGF表达、抑制肾间质成纤维细胞的增殖减轻RIF，保护肾脏功能。

The Protective Effect of Goralatide on Renal Interstitial Fibrosis of Unilateral Ureteral Obstruction(UUO)Rats

Objective Investigate the protective effect of goralatide on Renal Interstitial Fibrosis(RIF).Methods 15 male rats were randomly divided into 3 groups,including sham-operated group,unilateral ureteral obstructive(UUO) group without any treatment,UUO group treated with goralatide.Remove the ureteral obstruction side kidney after 15 days of post-operation,the amount of renal interstitial inflammation cells and the degree of RIF were observed by Masson staining and the expression of TGF-β1 and CTGF were observed by immunohistochemistry method.Results The index of urine protein in 24 h,creatine and urea nitrogen level in UUO group are all higher than those in sham-operated group and UUO group treated with goralatide(P<0.01);Compared with sham-operated group,the index of urine protein in 24 h,creatine and urea nitrogen level are increased in UUO group treated with goralatide(P>0.05).Compared with sham-operated group,the amount of renal interstitial inflammation cells were increased,RIF is significant,the expression of TGF-β1 and CTGF is higher than those in UUO group(P<0.01).Compared with UUO group,all the index are decreased in UUO group treated with goralatide but are still higher than those in sham-operated group(P<0.05).Conclusion Goralatide relieve RIF and protect renal function through decreased the expression of TGF-β1 and CTGF and restrain the proliferation of the Renal Interstitial fibroblasts.