| HPLC测定七味胃痛胶囊中胡椒碱的含量 |
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| 引用本文： | 靳占军,马文彪,陈珏蓓. HPLC测定七味胃痛胶囊中胡椒碱的含量[J]. 北方药学, 2012, 0(11): 1-1 |
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| 作者姓名： | 靳占军 马文彪 陈珏蓓 |
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| 作者单位： | 青海省格尔木市药品检验所,格尔木816000 |
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| 摘 要： | 目的:建立HPLC法测定七味胃痛胶囊中胡椒碱的含量,以有效控制该制剂胡椒碱的质量。方法:以InertsitC18柱(4.6mm×250mm,5um)为色谱柱;以甲醇-水(77∶23)为流动相;检测波长343nm;柱温30℃;流速1.0ml·min-1。结果:胡椒碱进样量在4.275～85.52μg·ml-1范围内线性关系良好(r=0.9998),平均回收率为98.4%,RSD为0.6%(n=5),样品在24h内稳定。结论:本文建立的含量测定方法简捷快速,结果准确可靠,可用于七味胃痛胶囊的质量控制。
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| 关 键 词： | 七味胃痛胶囊 胡椒碱 HPLC |
Determination of seven kinds of HPLC content of piperine in stomach capsule |
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| Jin Zhanjun Ma Wenbiao Chen Juebei. Determination of seven kinds of HPLC content of piperine in stomach capsule[J]. BEIFANG YAOXUE, 2012, 0(11): 1-1 |
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| Authors: | Jin Zhanjun Ma Wenbiao Chen Juebei |
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| Affiliation: | Jin Zhanjun Ma Wenbiao Chen Juebei(Golmud People＇s Hospital of Qinghai province 816000) |
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| Abstract: | Objective： To establish a method for determination of seven kinds of HPLC Weitong capsule of pipefine content, in older to effectively control the preparation of piperine quality. Methods： the Inertsit C18 column （4.6mm × 250mm,5um） is a chromatographic column; methanol - water（77：23 ）as mobile; detection wavelength：343nm ; column temperature30 degrees Celsius; velocity 1.0ml· min^-1. Results：Pepper alkali amount of sample in the 4.275N85.52μg· ml^-1 within a good linear relationship （1=0.9998）, the average recovery was 98.4%,RSD was 0.6%（n=5）,the sample in the 24h stability.Conclusion： the established method of determination the content of simple and rapid, accurate and reliable resuhs, can be used for the quality control of seven flavor capsule for treating stomachache. |
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| Keywords: | Seven taste Weitong capsule Pepper alkali HPLC |
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