In vivo autoradiography of [H]SCH 39166 in rat brain: selective displacement by D1/D5 antagonists |
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Authors: | Ruth A. Duffy Mary A. Hunt James K. Wamsley Robert D. McQuade |
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Affiliation: | a Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA;b Neuropsychiatric Research Institute, Fargo, ND, USA;c Department of Psychiatry, New York Medical College, Valhalla, NJ, USA;d Bristol-Myers Squibb, Princeton, NJ, USA |
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Abstract: | The purpose of this study was to examine the receptor occupancy of D1/D5 antagonists for D1-like dopamine receptors in rat brain using [3H]SCH 39166, a highly selective D1/D5 antagonist with low affinity for 5HT2 receptors. A single concentration of triated SCH 39166 was administered to rats, with or without competing doses of the D1/D5 antagonist SCH 23390 and unlabeled SCH 39166, the D2-like antagonists haloperidol or the 5-HT2 antagonist ketanserin. The bound radioactivity in the cortex, striatum, nucleus accumbens and olfactory tubercle was then quantified using an in vivo autoradiographic procedure. The results indicated that [3H]SCH 39166 was dose dependently displaced by the D1/D5 antagonists in regions associated with both the nigro-striatal pathway and the mesolimbic dopamine pathway, particularly the nucleus accumbens. Neither haloperidol nor ketanserin displaced [3H]SCH 39166 in any of the regions examined. The data were compared with previously published data examining the in vivo binding of [3H]SCH 39166 in rat brain homogenates. The relative values obtained were comparable to values detected in rat brain homogenates after in vivo binding of [3H]SCH 39166. |
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Keywords: | Dopamine D1 receptors Autoradiography SCH 39166 SCH 23390 Nigro-striatal pathway Mesolimbic pathway |
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