非清髓异基因外周血造血干细胞移植后供体细胞植入证据的研究

采用染色体荧光原位杂交（FISH）并结合常规染色体和染色体分带等方法，观察并比较了4例血液病人非清髓异基因外周血干细胞移植（NAPBSCT）后的植入情况。结果4例病人均有不同程度的植入，全部形成混合性嵌合体，其中2例转化为完全植入。统计结果分析表明，FISH分析中期分裂相与间期细胞的结果无统计学差异。与常规染色体检测结果相比较，FISH分析中期分裂相或间期细胞的植入率高，但无统计学差异。但FISH具有操作简单、实验周期短、结果敏感可靠等优点，适合用于性别不合的NAPBSCT后植入证据检测。

THE DETECTION OF THE EVIDENCE OF ENGRAFTMENT AFTER NONMYELOABLATIVE ALLOGENEIC PERIPHERAL BLOOD STEM CELL TRANSPLANTATION

This paper investigate the methods to detect engraftment rate of the four patients with hematological disorders who accepted nonmyeloablative allogeneic peripheral blood stem cell transplantation(NAPBSCT). To find out the best method, their engraftment rates were detected serially at different time after NAPBSCT by means of either FISH, or conventional chromosome analysis combined with R banding analysis concurrently.The results were carefully compared with one another. All these four sex mismatched cases were engrafted partially,and two of them changed to full engrafment. The results show no statistically significant difference in 3 groups (conventional method, FISH for hypermetaphase, FISH for interphase nuclei). But the results strongly indicate that FISH is a rapid, precise, objective,and reliable menthod for detection of the engraftment rate,and it is suitable for sex mismatched NAPBSCT.