四氯化碳诱发大鼠肝硬变过程中血清和肝组织透明质酸含量的变化

用四氯化碳诱发大鼠出现肝脏纤维化，采用ＥＬＩＳＡ方法检测大鼠血清和肝组织匀浆中透明质酸（Ｈｙａｌｕｒｏｎａｔｅ，ＨＡ）的含量，并分析ＨＡ与肝组织病理改变之间的关系。结果发现，血清ＨＡ含量在大鼠肝脏受损后早期即增加，并随肝组织损伤程度的加重而升高。肝组织内ＨＡ含量在肝硬变完全形成期时才明显高于正常组，与肝组织病理改变没有明显相关性。提示血清ＨＡ含量是早期判断肝细胞损伤和肝纤维化程度的有用指标。

Studies on Hyaluronate in Carbon Tetrachloride Induced Cirrhotic Rat Liver

Liver cirrhosis was induced in rats with carbon tetrachloride.Hyaluronate(HA)levels were measured in both serum and liver homogenate using ELISA.The results demonstrated a significant increase of serum HA levels that occurred early and in the late stage of liver fibrosis.The serum concentrations of HA in different stages paralleled the progressed liver damage.In contrast, no significant increase was seen in the liver homogenate level of HA in the early stage of liver fibrosis, but occurred in the late stage.The results suggest that immunoassay for HA in serum is a more sensitive index for liver cell damage and hepatic fibrosis.