少精子症、弱精子症和畸形精子症患者精子凋亡率比较

目的：比较少精子症、弱精子症和畸形精子症患者精子凋亡率。方法：应用计算机辅助精液分析（CASA）对生育组(n=14)、不育组［(n=48)，少精子症(精子密度<10×10^６•mL^-1)11例、弱精子症(a+b级精子百分率<50%)25例、畸形精子症(形态正常精子百分率<14%)12例］进行精液参数检测，瑞-姬染色检测精子凋亡率。结果：不育组及不育组中的少精子症组精子凋亡率均明显高于生育组（P<0.05）；弱、畸形精子症组精子凋亡〖JP3〗率与生育组比较差异均无显著性（P>0.05）。密度异常组(精子密度<20×10⁶•mL^-1)精子凋亡〖JP〗率明显高于密度正常组(精子密度≥20×10⁶•mL^-1)（P<0.05）；a+b级异常组(a+b<50%)精子凋亡率与正常组比较差异无显著性（P> 0.05）；精子形态异常组(形态正常精子<14%)凋亡率与正常组比较差异无显著性（P> 0.05）。结论：不育组精子凋亡率高于生育组，尤其是少精子症患者精子凋亡率增加更明显。

Comparisons of apoptotic rates of sperm between patients with oligozoospermia, asthenozoospermia and teratozoospermia

Objective To compare the apoptotic rates of sperm in patients with oligozoospermia,asthenozoospermia,teratozoospermia. Methods Semen parameters were determined by computer assisted sperm analysis (CASA). Semen samples were from 14 fertility persons and 48 infertility patients, of which 11 patients with oligozoospermia(sperm concentration<20×10~6·mL~ -1 ), 25 patients with asthenozoospermia (a+b<50%) and 12 patients with teratozoospermia(percentage of normal morphology sperm<14%). The apoptotic rates of sperm in two groups were analyzed by Wright-Giemsa staining. Results The apoptotic rates of sperm in infertility and oligozoospermia groups were significantly higher than that in fertility group (P<0.05). The apoptotic rates of sperm were not significantly different between asthenozoospermia and teratozoospermia groups (P>0.05). The apoptotic rate of sperm in abnormal sperm concentration (sperm concentration<20×10~6·mL~ -1 )group was significantly higher than that in nomal group (P <0.05). No significant difference of apoptotic rate of sperm of a+b grade motile sperm was found between the normal group and abnormal group (P >0.05). The apoptotic rate of sperm was not significantly different between the normal morphology sperm group and abnormal morphology sperm group (P >0.05). Conclusion The apoptotic rate of sperm in infertility group is significantly higher than that in fertility group, specially in oligozoospermia patients.