B10 细胞与非肥胖型糖尿病小鼠自身免疫性糖尿病发生的关系研究

目的：研究B10细胞与非肥胖型糖尿病小鼠自身免疫性糖尿病发生的关系。方法：选择20只6周龄NOD/LT雌性小鼠进行常规培养至30周龄，根据小鼠血糖、血肌酐和体重情况将小鼠分为自身免疫性糖尿病未发生组和发生组。利用酶联吸附反应检测两组小鼠脾脏组织中IL-10水平。利用流式细胞分选术检测两组小鼠脾脏组织汇总B10细胞比例。将40只6周龄NOD/LT雌性小鼠分为对照组和B10组，B10组小鼠接种分离得到的B10细胞，每周一次，对照组接种同等体积生理盐水，分别于第10、15、20、25、30周龄时检测小鼠自身免疫性糖尿病发生情况。结果：发生组小鼠血糖和血肌酐水平显著高于未发生组（ P ＜0.05），体重水平显著低于未发生组（ P ＜0.05）。发生组小鼠脾脏组织中IL-10水平显著高于未发生组。自身免疫性糖尿病发生组小鼠脾脏组织中B10细胞含量显著高于未发生组。当小鼠处于第10、15周龄时，B10组小鼠中自身免疫性糖尿病发生率显著低于对照组，但第20、25、30周时，B10组小鼠中自身免疫性糖尿病发生率显著高于对照组。结论： B10细胞的过度积累，可能是进一步诱发NOD小鼠自身免疫性糖尿病发生的原因之一。

Relationship between B10 cells and incidence of autoimmune diabetes in non obese diabetic mice

  Objective: To study relationship between B10 cells and the incidence of autoimmune diabetes in nonobese diabetic mice. Methods: 20 NOD/LT female mice of 6 week old were cultured in normal culture to 30 weeks,and the mice were divided into two groups according the mice′s blood glucose,serum creatinine and body weight detected at their 30 weeks old.IL-10 levels in spleen tissues of the two groups were detected by enzyme linked immunosorbent assay. We used flow cytometry to detect the proportion of B10 cells in the spleen of mice in the two groupsNOD/LT mice were randomly divided into control group and B10 group.The B10 cells were inoculated in B10 groups,their blood glucose were detected when they were 10,15,20,25 and 30 weeks old. Results: The blood glucose and serum creatinine levels were significantly higher in the group than that in the autoimmune diabetes group ( P < 0.05),and the body weight was significantly lower than that in the autoimmune diabetes group ( P < 0.05).The level of IL-10 in the spleen tissues of the autoimmune diabetes mice was significantly higher than that in the non autoimmune diabetes group. The content of B10 cells in the spleen of the mice with autoimmune diabetes mellitus was significantly higher than that in the non autoimmune diabetes groupWhen mice at the age of 10,15 weeks,the incidence of autoimmune diabetes in B10 group was significantly lower than that in the control group,but the incidence of autoimmune diabetes in B10 group was significantly higher than that in control group at 20,25 and 30 weeks. Conclusion:  The over accumulation of B10 cells may be one of the reasons for the further development of autoimmune diabetes in NOD mice.