急性白血病患者MSC的免疫原性及抑制异体T淋巴细胞增殖功能和机制研究

目的：研究分析急性白血病患者的骨髓间充质细胞（MSC）对异体T淋巴细胞增殖功能的抑制作用及其作用机制。 方法：选取30例确诊急性髓细胞白血病（AML组）患者及30例急性淋巴细胞白血病（ALL组），同期健康研究对象30例（健康组），分离培养三组研究对象的MSC细胞，测定三组MSC细胞培养上清液中细胞因子的表达水平，采用Transwell培养检测三组MSC对T淋巴细胞增殖的抑制作用，采用混合淋巴细胞方法检测MSC对异体T淋巴细胞增殖的抑制作用。 结果： AML组上清液中的TGF-β1、HGF水平显著低于ALL组和健康组（P<0.05），ALL组的IL-11水平显著高于AML组、健康组（P<0.05）；三组间MSC上清液中IL-6的水平差异无统计学意义（P>0.05）。AML、ALL、健康组的MSC细胞分泌的细胞因子对T淋巴细胞增殖仍然具有显著的抑制作用，接触性共培养和不直接接触的情况下对T淋巴细胞增殖的抑制作用差异无统计学意义（P>0.05）；在加入MSC后，三组的T细胞增殖作用受到显著的抑制，在加入抗TGF-β1抗体、抗HGF抗体后T细胞增殖抑制作用较单纯加入MSC时明显降低（P<0.05）。 结论：急性白血病患者的MSC对异体T淋巴细胞增殖具有抑制作用，其作用原理可能与细胞因子的分泌有关。

Study on immune function and mechanism of T lymphocyte proliferation in patients with acute leukemia MSC

 Objective: To study the inhibitory effect of bone marrow mesenchymal stem cells (MSC) on the proliferation of allogeneic T lymphocytes in patients with acute leukemia and its mechanism.Methods: 30 patients with acute myeloid leukemia (AML),30 patients with acute lymphoblastic leukemia (ALL),and 30 healthy subjects (healthy group) were selected.MSC cells were isolated and cultured in three groups.The expression level of MSC cells in three groups were determined by MSC assay.The inhibitory effect of MSC on the proliferation of T cells was detected by Transwell assay.Results: AML group supernatant TGF-β1,HGF levels were significantly lower than the ALL group and the healthy group (P<0.05),significantly higher than the levels of IL-11 ALL group of AML group,healthy group (P<0.05); three groups MSC supernatant IL-6 levels difference was not statistically significant (P> 0.05).AML,ALL,MSC cells secrete cytokines healthy group of T lymphocyte proliferation still had a significant inhibitory effect,inhibition of differences in the case of contact and not in direct contact co-culture of T lymphocyte proliferation was not statistically significant (P> 0.05); after joining the MSC,T cell proliferation in the three groups was significantly inhibited upon addition of anti-TGF-β1 antibody,anti-HGF antibody after T cell proliferation effective than a simple reversal join MSC (P<0.05). Conclusion: MSC for acute leukemia patients inhibit allogeneic T lymphocyte proliferation,functioning principle may be related to the secretion of cytokines.