miR-150 基因缺失对小鼠繁殖和血液学指标的影响

目的:探讨miR-150 基因缺失对小鼠繁殖和血液学指标的影响。方法:采用miR-150ko 小鼠观察该小鼠的窝产仔数和离乳率及生长曲线,测定其生殖指标;以全自动血球计数仪和自动生化分析仪测定2 月龄miR-150ko 小鼠和正常对照C57BL/6J 小鼠血液细胞学和血清生化参数。结果:miR-150ko 小鼠窝产仔数与离乳率与C57BL/6J 正常对照小鼠相比均显著下降;miR-150ko 小鼠外周血白细胞计数、中间细胞数、中性粒细胞数、中间细胞百分比、中性粒细胞百分比与C57BL/6J小鼠相比显著升高;而血小板计数、淋巴细胞百分比显著下降;miR-150ko 小鼠的血清葡萄糖、总胆固醇水平较正常对照小鼠显著增高。结论:miR-150 基因缺失可影响到小鼠的繁殖功能,并对某些血液细胞学指标及血糖、胆固醇水平产生影响。

miR-150 deletion affects breeding and hematologic parameters of mice

Objective:To investigate the effect of miR- 150 gene deletion on the breeding and hematologic parameters of mice.Methods: The nest litter size,wean rate and weight changes of miR-150 knock out (miR-150ko) and C57BL/6J mice were compared.The hematology indexes were analyzed by automated blood cell counter,the serum biochemical parameters were analyzed by automatic biochemical analyzer.Results: The nest litter size and wean rate of miR-150ko mice were significantly decreased compared with that of C57BL/6J mice.The number of total white blood cells,intermediate cells,neutrophils,and the percentage of neutrophils and intermediate cells were significantly increased in miR-150ko mice compared with that of C57BL/6J mice.However,the number and percentage of platelets and lymphocytes decreased significantly inmiR-150ko mice.In addition,the levels of serum glucose and TC were increased significantly in miR-150ko mice compared with that of C57BL/6J mice.Conclusion: miR-150 gene deletion impairs the breeding and has complex impact on hematologic parameters of mice.