伊马替尼诱导原代T细胞凋亡作用机制研究

目的：研究伊马替尼（IM）诱导正常人原代CD3⁺ T细胞凋亡的分子机制。 方法： 0～100 nmol/L的IM作用原代T细胞24 h后,应用流式细胞术检测细胞凋亡情况；应用实时荧光定量PCR技术检测Caspase-3、Caspase-8、A20和NF-κB基因表达变化；应用Western blot技术检测A20和NF-κB蛋白表达变化。结果：IM具有明显促T细胞凋亡能力，其凋亡分子Caspase-3 和A20基因表达水平均上调，而NF-κB的基因和蛋白水平均下调。结论：IM通过上调A20的表达诱导了T细胞的凋亡。

Mechanisms of imatinib mesylate induced apoptosis of primary T cells

Objective:To investigate mechanism of imatinib mesylate induced apoptosis of primary CD3⁺T cells.Methods: The CD3⁺T cells were stimulated by 0-100 nmol/L imatinib for 24 h,cell apoptosis was detected by flow cytometry;Caspase-3,Caspase-8,A20 and NF-κB expression levels were detected by Real-time quantitative PCR and Western blot.Results: IM significantly increased apoptosis of T cell;Caspase-3 and A20 gene expression levels were upregulated and NF-κB expression level was downregulated both in gene and protein levels.Conclusion: IM increased apoptosis of T cell by upregulating A20 expression.