去泛素化酶USP9X在结肠癌组织中的表达及意义

目的:探讨去泛素化酶USP9X在结肠癌组织中的表达特点及对结肠癌细胞增殖的影响。方法:收集解放军中部战区总医院病理科存档的结肠癌组织标本和癌旁正常结肠组织标本76例，免疫组化法检测USP9X在不同性别、年龄患者的结肠癌组织、癌旁正常结肠组织中的表达情况。siRNA抑制人结肠癌HCT116细胞株USP9X的表达，MTT法检测HCT116细胞增殖能力。结果:76例结肠癌患者中，USP9X阳性表达53例，占69.7%。癌旁正常大肠组织中，USP9X阳性表达率19.7%。USP9X的表达与结肠癌淋巴结转移和浸润深度相关。siRNA抑制结肠癌HCT116细胞USP9X表达后，与空白对照组和阴性对照组比较，干扰组细胞增殖能力显著下降。结论:去泛素化酶USP9X在结肠癌组织中的表达高于癌旁正常结肠组织，并与肿瘤侵袭转移相关。特异性抑制USP9X基因的表达可抑制结肠癌细胞的增殖能力。

The expression and significance of USP9X in colon cancer

Objective:To explore the expression of USP9X in colon cancer tissue and the effect of USP9X on proliferation of colon cancer cell.Methods:A total of 76 colon cancer patients who underwent surgery in general hospital of central war zone PLA was included.The expression of USP9X in colon cancer tissue and adjacent normal colon tissue was tested by immunohistochemistry.Then siRNA targeting USP9X was transfected into colon cancer HCT116 cell,and the proliferation ability of HCT116 cell was tested by MTT method.Results:There were 53 patients with USP9X positive cases among 76 colon cancer patients,and the positive expression rate was 69.7%.The expression of USP9X was found to be associated with lymph node metastasis and tumor invasion.After USP9X expression of HCT116 cell was suppressed by interference siRNAs,HCT116 cell proliferation was inhibited significantly.Conclusion:It was found that positive expression rate of USP9X in colon cancer tissue was higher than that in distal normal mucosa tissue.The increased expression of USP9X was probably associated with metastasis and tumor invasion.Downregulation of USP9X decreased the proliferation ability of colon cancer cell.