PLTP 在香烟诱导HBECs 合成IL-8 中的作用

目的:研究磷脂转运蛋白(PLTP)对香烟诱导人支气管上皮细胞(HBECs)合成白介素8(IL-8)的影响。方法:Wistar 大鼠连续3 d 被动吸烟,收集支气管肺泡灌洗液(BALF),苏木精-伊红(HE)染色,免疫组织化学法(IHC)检测蛋白表达;体外培养HBECs,不同浓度烟草提取物(CSE)刺激;PLTP siRNA 干扰后,进行CSE 刺激。MTT 法检测细胞增殖,同时检测IL-8 含量及PLTP 表达。结果:烟熏组BALF 白细胞计数以及分类计数较对照组均明显升高(P<0.01),IHC 显示烟熏组PLTP 和IL-8 蛋白表达高于对照组;高浓度CSE(2%、4%)抑制HBECs 增殖(P<0.001);不同浓度的CSE 作用24 h 后,IL-8mRNA 及蛋白表达量增加,而PLTP 蛋白表达量下降,并且IL-8 蛋白分泌呈现时间依赖性;PLTP siRNA 处理后,IL-8 表达量明显升高(P<0.001)。结论:PLTP 基因缺失促进香烟诱导HBECs 合成IL-8。

Effect of PLTP on CSE-induced IL-8 production in human bronchial epithelial cells

Objective:To investigate the effect of PLTP gene on CSE-induced IL-8 production in human bronchial epithelial cell line (HBECs)-Methods: Wistar rats were exposed to air or cigarette smoke for 6 hours/ day on 3 consecutive days,then the lungs were sectioned and examined-The number of total white blood cell and differential white blood cells in BALF were counted.The different concentrations of CSE co-cultured with HBECs for 24 hours.Cells growth was detected by MTT assay.Expression levels of PLTP mRNA and IL-8 mRNA were examined by RT-PCR,protein of PLTP was investigated by Western blot,and production of IL-8 ex-amined by ELISA.Results: The number of white blood cells in BALF was significantly increased compared with controls.Enhanced ex-pression level of PLTP and IL-8 were observed in CS.exposure group.Proliferation of HBECs tends to decrease at high concentrations of CSE(2.0% CSE and 4.0% CSE).The results suggested that the production of IL-8 induced by CSE in a time-and concentration-dependent manner,while the expression of PLTP induced by CSE in a dose-dependent manner.Furthermore,expression levels of IL-8 significantly increased after silence PLTP gene.Conclusion: PLTP siRNA could increase CSE.induced IL-8 production in HBECs-