Association of CTLA-4 gene polymorphism with Graves' disease and ophthalmopathy in Iranian patients |
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| Authors: | Alireza Esteghamati Omid Khalilzadeh Zahra Mobarra Mehdi Anvari Maryam Tahvildari Hoda Mojazi Amiri Armin Rashidi Ghasem Solgi Kazem Parivar Behrouz Nikbin Aliakbar Amirzargar |
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| Affiliation: | 1. Department of Epidemiology, Public Health College, Harbin Medical University, 157, Baojian Street, Nangang District, Harbin, Heilongjiang Province, PR China;2. Health Management College, Harbin Medical University, 157, Baojian Street, Nangang District, Harbin, Heilongjiang Province, PR China;3. Department of Immunology, Harbin Medical University, 157, Baojian Street, Nangang District, Harbin, Heilongjiang Province, PR China;1. Department of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan County, Taiwan;2. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan County, Taiwan;3. Clinical Informatics and Medical Statistics Research Center, College of Medicine, Chang Gung University, Taoyuan, Taiwan;4. Department of Pediatrics, Division of Hematology/Oncology, Chang Gung Children''s Hospital, Chang Gung University, Taoyuan County, Taiwan |
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| Abstract: | BackgroundThe cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene, is one of the candidate genes for susceptibility to Graves' disease. This study aimed to investigate the association of Graves' disease and Graves' ophthalmopathy with polymorphisms at position + 49 in exon 1 and positions ? 318 and ? 1147 in the promoter region of CTLA-4 gene in Iranian patients.MethodsA total of 205 unrelated Iranian patients with Graves' disease who were referred to the outpatient endocrine clinic of a large university general hospital and 103 sex-matched healthy controls were included in this study. Venous blood was obtained, genomic DNA was extracted by a salting out method, and the polymorphisms at positions + 49, ? 318 and ? 1147 of the CTLA-4 gene were determined using the PCR-restriction fragment length polymorphism method (PCR-RFLP). Genotype and allele frequencies were determined.ResultsThe frequency of the G allele at position + 49 was significantly higher in patients with Graves' disease than in the control group (27.1% vs. 15.1%, OR = 2.096, 95%CI = 1.350–3.253 and p < 0.01). Significant trends were not seen for the other two polymorphisms studied. In patients with ophthalmopathy, the frequency of the G allele at position + 49 was higher than in those without ophthalmopathy (33.8% vs. 20.0%, OR = 2.043, 95%CI = 1.304–3.202 and p < 0.01).ConclusionThe results of this study suggest that the G allele at position + 49 in exon1 of the CTLA-4 gene is associated with Graves' disease and Graves' ophthalmopathy in Iranian patients. |
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