低剂量纳曲酮对小鼠巨噬细胞的免疫调节作用及机理研究

目的:探讨纳曲酮(Naltrexone,NTX)对小鼠腹膜巨噬细胞表型,分泌细胞因子及吞噬功能的影响。方法:本实验利用新型四唑化合物比色法测得NTX 对RAW264郾7 细胞作用的最佳剂量;再将培养的腹膜巨噬细胞分为3 组,RPMI1640 空白对照组、脂多糖(LPS)阳性对照组和NTX 处理组,采用流式细胞术检测CD206、CD64 的阳性表达率及对葡聚糖的吞噬能力;ELISA 法检测白介素10(IL鄄10)、白介素6(IL-6)、白介素1茁(IL-1)、肿瘤坏死因子 (TNF )的分泌表达。结果:10-11 mol/ L 剂量可显著促进RAW264-7 细胞的增殖;NTX(10-11 mol/ L)处理可明显提高腹膜巨噬细胞表面CD64 的阳性表达,减少CD206 的表达;提高对葡聚糖的吞噬能力;TNF、IL-6、IL1的表达显著增加,IL-10 的表达无明显差异。结论:低剂量纳曲酮可改变巨噬细胞表型及功能,起免疫调节作用。

Immuno-regulatory effects on murine macrophages by low dose naltrexone(LDW)

 Objective:The effect of naltrexone on function of murine peritoneal macrophage in vitro was studied in order to illuminate its immune activity futher.Methods: Peritoneal macrophages were divided in three groups:RPMI1640 blank control group, LPS positive control group and NTX treated group.Various phenotypic and functional indices were tested by MTS,flow cytometry technology,phagocytosis experiment and ELISA.Results: Compared with RPMI1640 group,at a LDN exhibits paradoxical properties;the expression of CD64 on surface increased while the expression of CD206 decreased in NTX group; the expression of tumour necrosisfactor (TNF ), inter leukin-6 (IL-6),interleukin-1 (IL-1 )were increased.Conclusion: The results of experiment had proved that LDN could influence macrophage polarzation,regulate the inflamatory mediators production and affect the phagocytosis function ofperitoneal macrophage