miRNA-17-5p在胃癌患者血清中的表达及其临床意义

目的:探讨miRNA-17-5p在胃癌患者血清中的表达情况以及临床意义。方法:前瞻性选取54例胃癌患者及54例健康对照者纳入研究，抽取所有研究对象的血清标本，提取总RNA，逆转录后实时荧光定量PCR（qRT-PCR）检测miR-17-5p在血清中的表达水平，并分析miR-17-5p的表达与胃癌临床病理特征的关系。结果:miR-17-5p在胃癌患者血清中的表达量为0.67±0.11，显著低于对照组2.67±0.29，差异具有统计学意义（P<0.05）。胃癌患者血清 miR-17-5p的表达与胃癌患者的肿瘤大小、淋巴结转移及临床分期密切相关（P<0.05），但与患者年龄、性别及肿瘤分化程度无明显相关（P>0.05）。结论:miR-17-5p在胃癌患者血清中表达下调，且与患者临床病理指标密切相关，是胃癌临床诊断的有效分子指标。

Expression of circulating miR-17-5p in gastric cancer patients and its clinical significance

Objective:To explore the expression of miR-17-5p in the serum of gastric cancer patients and its clinical significance.Methods:Expression of miR-17-5p in serum of 54 gastric cancer patients and 54 healthy control persons was analyzed by qRT-PCR.Statistical analysis was performed to analyze the association between the miR-17-5p and the clinic pathological parameter.Results:The results of qRT-PCR showed that the circulating miR-17-5p was significantly down-regulated in the serum of the patients with gastric cancer patients compared to the normal controls.And the decreased expression of miR-17-5p was associated with tumor size,lymph node metastasis and TNM stage,but not significantly correlated with age,gender and tumor differentiation.Conclusion:Circulating miR-17-5p expression is significantly down-regulated in serum of gastric cancer patients,and is a promising diagnostic and prognostic biomarker for gastric cancer patients.