COPD 患者Th17 细胞和Treg 细胞介导的免疫应答变化及免疫失衡与肺功能的关系研究

目的:探讨慢性阻塞性肺疾病(COPD)患者外周血Th17 细胞和调节性T 细胞(Treg)及其介导的免疫应答变化与患者肺功能的关系。方法:选取本院呼吸内科收治的90 例COPD 患者(COPD 组)和同期年龄、性别基本匹配的45 例健康体检对象作为对照组,并根据COPD 病情进行亚组分析,分别测定各组研究对象肺功能指标、Th17 细胞、Treg 细胞及血清炎症因子水平。结果:在COPD 患者间,随着病情加重,FEV1%、FVC%、FEV1/ FVC 比值中度COPD 组<重度COPD 组<极重度组(P<0.05);在COPD 患者间,随着病情加重,外周血中Th17 细胞、Th17/ Treg 比值中度COPD 组<重度COPD 组<极重度组(P<0.05),外周血Treg 细胞比值中度COPD 组>重度COPD 组>极重度组(P<0.05),且外周血Th17 细胞、Treg 细胞相关细胞因子变化具有一致性;COPD 患者的肺功能指标FEV1%、FVC%、FEV1/ FVC 与外周血中Th17 细胞、Th17/ Treg 比值呈显著的负相关关系(P<0.05),肺功能指标FEV1%、FVC%、FEV1/ FVC 与外周血Treg 比值呈显著的正相关关系(P<0.05)。结论:COPD外周血Th17/ Treg 比值与患者肺功能下降具有显著的相关性。

Relationship of immune response and immune imbalance mediated by Th17 cells and Treg cells with pulmonary function in COPD patients

Objective:To investigate the relationship of the immune response and immune imbalance mediated by Th17 cells and Treg cells with pulmonary function in chronic obstructive pulmonary disease (COPD) patients.Methods:90 COPD patients and 45 healthy volunteers with matched age and sex were selected.The COPD patients were divided into 2 groups according to the patient's condition.45 healthy volunteers were collected as the control group.The lung function, Th17 cells, Treg cells and serum levels of in-flammatory cytokines were detected and compared.Results:In COPD patients, the FEV1%, FVC%, FEV1/ FVC, Th17,Th17/ Treg and inflammatory cytokines had increased in an order of moderate COPD, severe COPD and very severe COPD (P<0.05).Treg had increased in an order of very severe COPD, severe COPD and moderate COPD (P <0.05).FEV1%, FVC% and FEV1/ FVC were negatively correlated with Th17 and Th17/ Treg (P<0.05).FEV1%, FVC% and FEV1/ FVC were positively correlated with Treg (P<0.05).Conclusion:Th17/ Treg in peripheral blood of COPD patients is significantly correlated with the decrease of pulmonary function.