pre-miR-27a单核苷酸多态与非吸烟女性肺癌发生的相关性

目的:探讨miR-27a(rs895819，A>G)多态与非吸烟女性肺癌发生的相关性。方法:采用病例-对照研究方法，使用PCR-RFLP技术检测90例非吸烟女性原发性肺癌患者和97例非吸烟女性体检者rs895819位点基因型分布频率。非条件Logistic统计分析不同基因型与肺癌发生的相关性，并分析该多态与烹饪油烟暴露的协同效应及与临床参数的相关性。结果:肺癌组rs895819 位点GG突变基因型和G等位基因频率明显高于对照组(P=0.047,P=0.016)。AG+GG基因型明显增加了非吸烟女性肺癌的发生风险(OR=1.82，95%CI=1.01～3.28，P=0.038)。肺癌组和对照组烹饪油烟暴露率分别为57%和42%，差异有统计学意义(P=0.035)。回归分析显示该多态位点与烹饪油烟无交互作用；与临床病理特征(病理类型，临床分期和淋巴结转移)均无相关性。结论:has-miR-27a rs895819 G等位基因增加了非吸烟女性肺癌发生的风险。

Association of miR-27a(rs895819) polymorphism with lung cancer risk in non-smoking female of Beijing area

Objective:To investigate the association of miR-27a rs895819 variant and risk of non-smoking female lung cancer(LC)in a Chinese Han population.Methods:In the case-control study,genotype distribution of rs895819 in 90 LC cases and 97 age-matched healthy check-up controls were determined by polymerase chain reaction-restriction fragment length polymorphism assay(PCR-RFLP).The correlation of rs895819 SNPs with the susceptibility to LC was analyzed.Results:The frequencies of the GG genotype and G allele of rs895819 variant were significantly higher in the LC group than that in the control group(P=0.047,P=0.016).Genotype AG+GG showed a significantly increased risk of LC(OR=1.82,95%CI=1.01~3.28,P=0.038).There was significant difference between LC and control group for cooking oil fume exposure(P=0.035).However,The combination of rs895819 SNP and cooking oil fume exposure had no significant influence on non-smoking female LC risk.No significant association was found between rs895819 SNP and clinical pathological parameters by stratified analysis.Conclusion:Allele G within rs895819 was significantly associated with lung cancer risk in a Han non-smoking female population of Northern China.