人脐带血干细胞移植对2 型糖尿病家兔血清学的影响

目的:观察人脐带血干细胞(Human umbilical cord blood stem cells,HUCBSC)移植后2 型糖尿病家兔血糖、胰岛素及二肽基肽酶郁( Dipeptidyl peptidase ,DPP / CD26 )的变化。方法:18 只家兔随机分成正常对照组(6 只,C 组)和糖尿病造模组(12 只),造模组制备2 型糖尿病模型,其中6 只经兔耳缘静脉输注给予免疫学表型为CD45+ 、CD34- 的HUCBSC移植(A 组),6 只同法输注等量的PBS 液作为对照(B 组)。以上三组家兔均喂养观察4 周,每天监测家兔血糖,每周测血胰岛素及DPP / CD26 的水平。结果:HUCBSC 阳性标志物CD45 表达率达100%,阴性标志物CD34 表达率为3.5%。与非移植组相比,HUCBSC 移植组家兔血糖和DPP / CD26 逐渐下降,胰岛素水平逐渐上升,差异均有统计学意义(P<0.01)。结论:HUCBSC 呈圆形或椭圆形贴壁生长,免疫学表型为CD45+ 、CD34- 。HUCBSC 移植可以明显降低家兔血糖、增加胰岛素分泌量、降低血DPP / CD26 的水平,从而为临床糖尿病及其并发症的治疗提供新的理论依据。

Effection of human umbilical cord blood stem cell transplantation on serum of rabbits with type 2 diabetes mellitus

Objective:To observe the changes of blood glucose,insulin and dipeptidyl peptidase (DPP / CD26)on type 2 diabetes mellitus in rabbits after HUCBSC(human umbilical cord blood stem cells)transplantation.Methods: 18 rabbits were randomly divided into normal control group (6 rats,Group C) and diabetic model group (12 rats).After preparation model of type 2 diabetes,and 6 rats of them were treated with HUCBSC (CD45+ ,CD34- ) transplantation by ear vein transfusion (Group A),and 6 rats were treated with PBS(Group B).All three groups of rabbits were fed for 4 weeks,and the blood glucose was monitored every day,and the level of blood insulin and DPP-IV/ CD26 were measured every week.Results: The negative expression rate of CD34 in HUCBSC was 96.5%.The positive expression rate of CD45 in HUCBSC was 100%.Compared with non transplantation group,the blood glucose and DPP-IV/CD26 in the umbilical cord blood stem cell transplantation group were gradually decreased,and insulin level was gradually increased,the difference was statistically significant (P<0.01).Conclusion: HUCBSC were round or oval,with adherent growth,HUCBSC tranplantation can significantly reduce blood glucose,increase insulin secretion,reduce the level of DPP-IV/ CD26,the immunological phenotype of HUCBSC was CD45+ ,CD34- ,thus providing a new theoretical basis for the clinical treatment of diabetes and its complications.