冠心康对实验性大鼠急性心肌缺血的保护作用

目的:探讨冠心康颗粒对大鼠急性心肌缺血的保护作用及其机理。方法:雄性大鼠32只,随机分为4组,每组8只,预防给药7d。灌胃结束后,采用在体大鼠冠状动脉左前降支结扎的方法建立心肌缺血模型,观察冠心康颗粒对大鼠急性心肌缺血后心肌中NO和NOS含量的影响,用免疫组化法检测Bc l-2蛋白在心肌中的表达。结果:模型组、冠心康组、地奥组与假手术组比较,心肌组织NO及NOS含量均有明显降低(P<0.05);而与模型组相比,冠心康组、地奥组的NO及NOS含量均有明显的恢复(P<0.05),提示冠心康颗粒可明显提高大鼠急性心肌缺血后心肌中NO和NOS含量(P<0.005,0.005),同时增加Bc l-2蛋白的表达,减少细胞凋亡。结论:冠心康颗粒对大鼠急性心肌缺血具有保护作用,可能与其提高心肌中NO和NOS含量,增加Bc l-2蛋白的表达有关。

Protective Effect of Guanxinkang Capsule against Myocardium Injury Induced by Acute Ischemia in Rats

Objective:To explore the effect and mechanism of Guanxinkang Capsule against myocardium injury induced by acute ischemia in rats.Methods:32 male rats were randomly divided into 4 groups,8 in every group.After fedding Guanxinkang Capsule for 7 dags preventedly,to ligate forward-descending branch of left coronary artery,establish the injured model and observe the changes of NO and NOS of myocardium.The myocardial expression of Bcl-2 was detected by immunohistochemistry.Results:NO and NOS of myocardium apparentedly decreased in model group,Guanxinkang Capsule group and Diao group(P<0.05).NO and NOS of Guanxinkang Capsule group and Diao group apparentedly recovery to model group(P<0.05).This prompted that Guanxinkang Capsule could increase NO and NOS of myocardium in ischemia rats(P<0.005,0.005) and increase myocardial Bcl-2 protein expression,inhibit myocardial apoptosis.Conclusion:the effect of Guanxinkang Capsule against myocardium injury induced by acute ischemia in rats may have relationship with increasing NO and NOS of myocardium and expression of Bcl-2 protein.