复方山楂饮对实验性二硫化碳毒性视网膜损害防护作用的组织学研究

目的 探讨复方山楂饮对二硫化碳(carbon disulfide CS2)毒性视网膜损害的防护作用。 方法 30只健康新西兰白兔随机分为正常对照组、染毒对照组及治疗组3组。染毒及治疗组用CS2吸入式染毒,每日连续3小时,每周连续6天,共3周。治疗组在染毒前口服复方山楂饮8.3ml/kg。实验3周后取兔眼视网膜组织作光镜及透射电镜检查。 结果 光镜及电镜均显示染毒对照组视网膜各层细胞均呈明显变性改变。光镜显示染毒对照组视细胞内外段结构破坏,神经纤维层疏松,神经节细胞空泡变性。电镜显示染毒对照组视细胞突触空泡化改变,突触后间隙加大,突触小棒消失。治疗组视网膜各层细胞结构大致正常。 结论 复方山楂饮可提高CS2染毒兔对CS2的耐受能力,对视网膜具有一定程度的保护作用。 (中华眼底病杂志, 1999, 15: 249-252)

Histological study of haw drink compound in protecting against experimental carbon disulfide toxic damage of relina

Purpose To investigate the protective effect of“haw drink compound”against carbon disulfide (CS2)toxic retinal damage． Methods Thirty healthy white adult New Zealand rabbits were divided at random into 3 groups:the normal control group,the exposure control group and the treatment group．The experimental rabbits were contaminated by inhaling CS2 for 3 continuoues hours in 6 consecutive days in a week for totaly 3 weeks．The rabbits in the treatment group were given haw drink compound before containation．After 3 weeks of the experiment,the retinal tissues of the rabbits were examined with light microscope and transmissing electronmicroscope(TEM). Results The ultrastructures of the retinal tissues of the exposure control group were more abnormal than those of the treatment group and the normal control group．TEM showed that every layer cell of the retinal in the exposure control group showed apparent degenerative changes ,but that in the treatment group showed apparent degenerative changes,but that in the treatment group was nomal．The light microscpic picture showed that the inner segments and the outer segments of photoreceptors construction were destroyed,the nerve fiber layer was loose and ganglion celles vacuolated in the retinat of the exposure control group．TEM showed that The photorecepter synapses were vacuolated,the postsynaptic space became wider and the synaptic sticks disappered． Conclusion How drink compound can improve the tolerance to CS2 toxicity in inducing the retinal damage of rabbits. (Chin J Ocul Fundus Dis, 1999, 15: )