Notch-1和Notch-2在卵巢肿瘤中的表达及意义

目的 探讨Notch-1和Notch-2在卵巢肿瘤的形成和转化中的表达特点及其潜在肿瘤生物学意义。方法 2000．12—2003-06采用人类卵巢肿瘤组织芯片和免疫组织化学染色方法，在完全相同的实验条件下检测各类卵巢肿瘤Notch-1和Notch-2的表达和活化情况。结果 Notch-1在50％（3／6）的浆液性囊腺瘤和33．3％（2／6）的粘液性囊腺瘤中有核易位现象；其中，16．7％（1／6）和33．3％（2／6）属于明显易位（＋＋／＋＋＋）。在交界性肿瘤中的核易位无论是频率（20％；1／5）还是易位的阳性程度均明显减低，而这种趋势在各类卵巢恶性肿瘤中亦可见到。Mann Whitnoy U检验显示：Notch-1的核易位在良性和恶性肿瘤之间差异有统计学意义（P〈0．05）。Notch-2普遍存在于各类卵巢上皮肿瘤中；其表达水平和核易位情况与肿瘤的良恶性之间无关。结论 Notch-1的表达异常和核易位出现在卵巢上皮的恶性转化的过程，它可能是卵巢肿瘤恶变风险和不良预后的生物学标志物。

Patterns of Notch - 1 and Notch - 2 expression in ovarian tumors and their oncological significance

Objective To study the expression and nuclear translocation of Notch-1 and Notch-2 in different ovarian tumors and to analyze its clinical biological significance in tumor transformation and progression. Methods The ovarian tumor array-based immunocytochemical staining was performed to check the expression patterns and intracellular distribution of Notch-1 and -2 in ovarian tumors under the same experimental condition. Results The rates of Notch-1 nuclear translocation were 50%(3/6)and 33.3%(2/6)in serous and mucinous cystadenoma respectively, among which 16.7% (1/6) and 33.3% (2/6) showed more distinctively( / ). In contrast, the borderline and malignant tumors exhibited downregulated Notch-1 expression as well as reduced and even diminished nulear translocation (P<0.05 ). Notch-2 expression and nuclear translocation could be observed either in benign tumors or in borderline and malignant ones (P>0.05). Conclusion The aberrant Notch-1 but not Notch-2 expression occurs in the transformation phase of ovarian tumors, indicating its value in the evaluation of transformation risk and poor prognosis of ovarian tumors.