银黄液对大鼠膀胱黏膜急性损伤保护作用的实验研究

目的探讨银黄液对大鼠膀胱黏膜急性损伤的保护作用及黏膜损伤后修复的影响。方法将32只SPF级成年雌性SD大鼠随机分为A、B、C、D共4组，每组8只，A组为正常对照组，B组为模型对照组，C组为治疗组，D组为预防治疗组。膀胱内灌注盐酸制作膀胱黏膜损伤的动物模型,并在建模前或建模后膀胱内灌注银黄液。通过HE染色、组织学损伤评分和膀胱组织中HSP70的表达评估各组炎症严重程度，并分析与黏膜受损程度的关系。结果光镜下病理改变，B组有大量炎性细胞浸润、毛细血管明显扩张，上皮细胞坏死。C组少量的炎性细胞浸润、毛细血管扩张。D组少量炎性细胞浸润，无或仅有少量炎性细胞浸润；组织学损伤评分，A组组织学损伤轻微，B组评分明显高于C、D组(P<0.01)，C组评分高于D组(P<0.05)；HSP70的表达，A组HSP70表达程度明显低于B、C、D组(P<0.01)，B组表达明显高于C、D组(P<0.01)，C组表达高于D组(P<0.05)。结论银黄液可以提高膀胱黏膜抗急性化学性损伤作用以及促进损伤后的膀胱黏膜修复。

Experimental Study on Yinhuang Solution on Bladder Mucosal Injury in Rat

ObjectiveTo investigate the effects of Yinhuang solution on the bladder mucosal before and/or after mucosal injury.Methods32 SPF female SD rats were randomly divided into 4 groups with 8 rats in each group. Group A was used for the normal control group, group B for the model control group, group C for preventive group, group D for preventive and therapeutic group. Injured rats were created by hydrochloric acid applied on bladder mucosa. After the models were established or before and after the models were established, bladders were perused with Yinhuang solution. Severity of inflammatory infiltration were observed by HE staining, histological score and heat shock protein 70 (HSP70) expressed in the mucosa.ResultsEndoscopic pathological change showed that group B had a large number of inflammatory cell infiltration, significant expansion of capillary, epithelial cell necrosis. Group C had a small amount of expansion of capillary, inflammatory cell infiltration. Group D scattered in expansion of capillary, a small amount of inflammatory cell infiltration. Concerning the results of histological score and HSP70 expression, there was a significant difference between group A and groups B, C or D (P<0.01); there was a significant difference between group B and groups C or D (P<0.01); there was a significant difference between group C and group D (P<0.05).ConclusionYinhuang solution can evidently improve the repair capability of bladder mucosa after injury.