基于网络药理学探讨金银花抗动脉粥样硬化的作用机制

目的 运用网络药理学方法探究金银花治疗动脉粥样硬化的分子作用机制。方法 通过多个数据库查找并筛选金银花的活性成分、作用靶点和动脉粥样硬化基因。通过Cytoscape 3.7.2软件构建“药物-活性成分-靶点-疾病”网络。运用R4.0.4软件进行GO功能分析及KEGG通路分析。结果 筛选出17个活性成分，196个靶标蛋白，2419个疾病基因，129个药物-疾病交集基因。GO功能富集分析得到GO条目2584个，包括生物过程2328个，分子功能172个和细胞组分84个。KEGG通路富集筛选得到信号通路162条，主要涉及血脂和动脉粥样硬化、流体剪切应力和动脉粥样硬化等。结论 金银花可能主要通过β-胡萝卜素、木樨草素、山柰酚和槲皮素等多种活性成分作用于JUN、RELA、IL1B、IL6和MAPK1等基因，直接调控血脂和动脉粥样硬化以及流体剪切应力和动脉粥样硬化通路起到抗动脉粥样硬化作用。

Discussion on the Mechanism of Anti-Atherosclerosis of Flos Lonicerae Based on Network Pharmacology

Objective Using the method of network pharmacology to explore the molecular mechanism of Flos Lonicerae in the treatment of atherosclerosis.Methods The active components,action targets and atherosclerosis genes of Lonicerae japonica were searched and screened through multiple databases.PPI network and drug-active component-targets-disease network were mapped by Cytoscape 3.7.2 software.Functional enrichment and main action pathway analysis were carried out through R 4.0.4 software.Results 17 active ingredients,196 target proteins,2419 disease genes and 129 drug-disease intersection genes were screened out.A total of 2584 GO items were obtained by GO functional enrichment analysis,including 2328 biological processes,172 molecular functions and 84 cellular components.A total of 162 signaling pathways were obtained by KEGG pathway enrichment and screening,mainly involving Lipid and atherosclerosis,Fluid shear stress and atherosclerosis,etc.Conclusions Flos Lonicerae may directly regulate blood lipid and atherosclerosis as well as fluid shear stress and atherosclerosis pathway by acting on genes such as JUN,RELA,IL1B,IL6 and MAPK1 mainly throughβ-carotene,luteolin,kaumferol and quercetin.