| RhoA/Rho激酶信号通路对小鼠抗衰老Klotho蛋白表达的影响 |
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| 引用本文： | 于凤芹,彭海,杨艳飞,邓刚,李敏. RhoA/Rho激酶信号通路对小鼠抗衰老Klotho蛋白表达的影响[J]. 卒中与神经疾病, 2010, 17(1): 3-5,22 |
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| 作者姓名： | 于凤芹 彭海 杨艳飞 邓刚 李敏 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院神经内科,武汉,430022
2. 湖北省宜昌市第二人民医院神经内科 |
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| 摘 要： | 目的观测RhoA/ROCK信号通路对小鼠脑脉络丛Klotho(KL)蛋白表达的影响。方法将24只昆明小鼠随机分为3组,即对照组,一氧化氮合酶(Nitric Oxide Synthesis,NOS)抑制组(L-NAME组)和L-NAME+法舒地尔组,连续处理4周。用免疫组织化学方法观察KL蛋白表达;Western blot检测KL蛋白表达水平的变化;RT-PCR检测RhoA和ROCK mRNA表达水平。结果与对照组比较,L-NAME组ROCK mRNA表达水平升高(P0.01),KL蛋白表达水平明显降低(P0.01)。给予ROCK阻滞剂法舒地尔后ROCK mRNA表达水平降低(P0.01),Kl蛋白表达水平明显增高(P0.01)。结论L-NAME所致KL蛋白表达下调与RhoA/ROCK信号通路活性增强有关。
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| 关 键 词： | RhoA/Rho激酶 小鼠 Klotho蛋白 法舒地尔 |
Effect of RhoA/Rho-kinase on the expression of anti-aging klotho protein in the brain of mouse |
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| Yu Fengqin,Peng Hai,Yang Yanfei,et al. Effect of RhoA/Rho-kinase on the expression of anti-aging klotho protein in the brain of mouse[J]. Stroke and Nervous Diseases, 2010, 17(1): 3-5,22 |
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| Authors: | Yu Fengqin Peng Hai Yang Yanfei et al |
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| Affiliation: | Yu Fengqin,Peng Hai,Yang Yanfei,et al.Department of Neurology,Xiehe Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430022 |
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| Abstract: | Objective To investigate the effect of RhoA/Rho-kinase pathway on the expression of Klotho(KL) protein in the brain choroid plexus in mouse.Methods 24 male Kunming mice were divided randomly into three groups: the normal control group,NOS blockade(L-NAME) group,and L-NAME plus fasudil group,and were administered by corresponding medicine respectively,for four weeks successively.The expression of KL protein in the brain choroid plexus were measured by immunohistochemistry and Western blotting.The expression ... |
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| Keywords: | RhoA/Rho-kinase Mouse Klotho protein Fasudil |
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