| HPLC法测定枇杷核中苦杏仁苷含量 |
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| 引用本文： | 林国荣,沈高杨,陈剑锋. HPLC法测定枇杷核中苦杏仁苷含量[J]. 海峡药学, 2007, 19(1): 41-42 |
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| 作者姓名： | 林国荣 沈高杨 陈剑锋 |
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| 作者单位： | 莆田学院环境与生命科学院,莆田,351100;福州大学生物科学与工程学院,福州,350002 |
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| 摘 要： | 目的建立常用中药枇杷核中苦杏仁苷含量的测定方法。方法色谱柱Hypersil ODS C18（4．0mm×250mm，5μm），流动相：甲醇-水（20：80）、检测波长215nm。结果苦杏仁苷在7．0μg～70．0μg·mL^-1成良好线性关系，回归方程为Y=132．38X=102．37，r=0．9995，平均回收率为100．17％。结论本方法分离效果好，重现性好，操作简便，可用于制定枇杷核的质量标准。
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| 关 键 词： | 枇杷核 HPLC 苦杏仁苷 |
| 文章编号： | 1006-3765（2007）01-0041-02 |
Content determination of amygdalin in eriobotrya japonia nut by HPLC |
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| LIN Guo-rong,SHEN Gao-yang,CHEN Jian-feng. Content determination of amygdalin in eriobotrya japonia nut by HPLC[J]. Strait Pharmaceutical Journal, 2007, 19(1): 41-42 |
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| Authors: | LIN Guo-rong SHEN Gao-yang CHEN Jian-feng |
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| Affiliation: | 1. Deparment of environment and life science of PuTian college, Putian, 351100, China;2. College of Biological Science and Technology of Fuzhou University, Fuzhou, 350002, China |
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| Abstract: | OBJECTIVE To set up a HPLC method for the determination of the content of amygdalin in unsually eriobotrya japonia nut. METHODS Chromatographic column Hypersil ODSC18 （4.0mm × 250mm, 5μm）was used ,mobil phase was methanol-water（20：80） ,and the content was detected at the UV wavelength of 215nm. RESULTS The linear range of amygdalin was 7.0-70. 0μg·mL^-1 （r=0. 9995）,an average recovery of samples was 100. 17%. CONCLUSION This method is simple, separated completely and good reproducibility,and can be used as a quantity control standard in eriobotrya japonia nut. |
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| Keywords: | eriobotrya japonia nut HPLC Amygdalin |
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