穴位注射黄芪对哮喘大鼠p38MAPK及Th1/Th2因子的影响

目的 观察哮喘大鼠肺组织p38蛋白激酶（p38 MAPK）和Th1/Th2类细胞因子（IFN-γ/IL-4）表达的变化,探讨穴位注射黄芪对其影响及可能机制.方法 SD大鼠随机分成4组（10只/组）,即正常对照组、哮喘模型组、地塞米松组和黄芪穴位注射组.应用卵蛋白（OVA）致敏激发法制备哮喘大鼠模型,分别采用酶联免疫吸附法（ELlSA）和蛋白质印迹检测血清中IL-4、IFN-γ含量和磷酸化p38 MAPK的变化,并观察肺组织病理学改变.结果 ①与正常对照组相比,哮喘模型组大鼠肺组织磷酸化p38 MAPK表达水平升高,血清IL-4含量升高、IFN-γ含量降低（P＜0.05）;②与哮喘模型组相比,黄芪穴位注射组大鼠血清IL-4含量、磷酸化p38 MAPK表达水平降低,血清IFN-γ含量升高（P＜0.05）;③黄芪穴位注射组与地塞米松组比较血清IL-4含量升高,IFN-γ含量降低,磷酸化p38 MAPK表达水平降低,差异有统计学意义（P＜0.05）;④在肺组织病理学方面,穴位注射黄芪注射液能明显改变哮喘大鼠病理变化;⑤肺组织磷酸化p38 MAPK的表达与IL-4比较呈显著正相关（r=0.596,P＜0.05）,与IFN-y比较呈显著负相关（r=-0.634,P＜0.05）.结论 p38 MAPK可能调控支气管哮喘的发病机制;穴位注射黄芪与糖皮质激素比较,对哮喘大鼠具有保护作用,偏重不一,其治疗作用与抑制p38 MAPK磷酸化、下调IL-4、上升IFN-γ表达水平有关.

Effccts of acupoint injection of astragalus on the expression of p38 mitogen-activated protein kinase and Th1/Th2 cytokines in asthma rats

Objective To observe the expression of p38 mitogen-activated protein kinase （p38MAPK） and Th1/Th2 Cytokines in lung tissue of asthmatic rats,and explore the effects and possible mechanism treated by astragalus in acupoint injection.Methods Forty SD rats were randomly divided into four groups （10/ group）：normal control group,asthma model group,dexamethasone group,acupoint injection of astragalus group.In the experiment,the asthmatic rat models were set up by the ovalbumin （OVA） sensitized methods.,The level of IL-4 and IFN-γ in serum and the expression of phospho-p38MAPK in lung tissue were respectively tested by enzyme-linked immunosorbent assay （ELISA） and Western blot.Results 1.Compared with norual control group,the level of IL-4 in serum and the expression of phospho-p38MAPK in lung tissue were significantly higher in asthma model group,while IFN-γ was lower （P ＜ 0.05）.2.Compared with asthma model group,the level of IL-4 in serum and the expression of phospho-p38MAPK in lung tissue were significantly lower in acupoint injection of astragalus group,while IFN-γ was higher （P ＜ 0.05）.3.The level of IL-4 in serum in acupoint injection of astragalus group was higher than that of dexamethasone group,while the expression of phospho-p38MAPK and the level of IFN-γ in serum were in contrast （P ＜ 0.05）.4.Acupoint injection of astragalus could significantly alleviate the histopathological damage of lung tissue in asthmatic rats.5.There were positive correlations between the expression of phospho-p38 MAPK and the level of IL-4 in serum （r =0.596,P ＜ 0.05）,and negative correlations between the expression of phospho-p38 MAPK and the level of IFN-γ in serum （r =-0.634,P ＜ 0.05）.Conclusion p38MAPK may play a role in pathogenesis of asthma.Compared with glucocorticoid,acupoint injection of astragalus could make an effect on the protection of asthmatic rats which may be associated with the inhibiting the expression of phospho-p38 MAPK,the depression of IL-4 and the improvement of IFN-γ,while the effects on the level of cytokines and the expression of phospho-p38 MAPK may be different.