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Effect of nickel sulfate on cellular proliferation and Epstein-Barr virus antigen expression in lymphoblastoid cell lines
Authors:Y T Wu  H L Luo  D R Johnson
Affiliation:1. Department of Cancer Etiology, Cancer Institute, Zhongshan University of Medical Sciences, Guangzhou People''s Republic of China;2. Department of Pathology and Microbiology, University of Nebraska Medical Center, 42nd & Dewey Avenue, Omaha, NE 68105 U.S.A.;3. Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 42nd & Dewey Avenue, Omaha, NE 68105 U.S.A.;1. Doctoral Candidate, Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou, Fujian, China;2. Physician, Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China;3. Master Degree Candidate, Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou, Fujian, China;4. Physician, Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China;6. Professor, Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou, Fujian, China;5. Associate Professor, Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou, Fujian, China;1. Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, WI, United States;2. Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, United States;3. Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, United States;4. Department of Medical Oncology, Medical College of Wisconsin, Milwaukee, WI, United States;5. Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, Saint Louis, MO, United States;1. Institut Ecocitoyen pour la Connaissance des Pollutions - Centre de Vie La Fossette, RD 268, 13270 Fos-sur-Mer, France;2. University of Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC, 38000 Grenoble, France;3. University of Rennes, Agrocampus Ouest, CNRS, UMR 6625 IRMAR, F-35000 Rennes, France;1. Department of Medicine DIMED, University of Padova, Padova, Italy;2. Department of Neuroscience DNS, Otolaryngology Section, University of Padova, Padova, Italy;3. Technology for Health (PhD program), Department of Information Engineering, University of Brescia, Brescia, Italy;4. University Health Network (UHN) Guided Therapeutics (GTx) Program International Scholar, UHN, Toronto, Canada;5. Artificial Intelligence in Medicine and Innovation in Clinical Research and Methodology (PhD program), Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy;6. Department of Cardiac-Thoracic-Vascular Sciences and Public Health, University of Padova, Padova, Italy
Abstract:Nickel is found in high levels in the environment of the high-risk areas for nasopharyngeal carcinoma (NPC) of China. Epstein-Barr virus (EBV) is associated with NPC and the interaction of nickel and EBV may be a contributive cofactor to the development of NPC. The study of the in vitro effect of nickel sulfate on cell proliferation and EBV-antigen expression demonstrated that nickel increases cell proliferation of some EBV-positive lymphoblastoid cell lines and increases early antigen expression of Raji cells. Nickel exerted variable effects on viral capsid antigen (VCA): increasing VCA-positive cells in B95-8 cells while decreasing VCA in P3HR-1 cells. It is proposed that the uptake of nickel in NPC high-risk areas could be one of the factors responsible for cancer development in the nasopharynx in China.
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