阿仑膦酸钠对兔破骨细胞功能的影响

用骨陷窝形成分析法观察阿仑膦酸钠对体外培养破骨细胞功能的影响，探讨可能的作用机制。在建立兔破骨细胞培养方法的基础上，用不同浓度的阿仑膦酸钠分别与骨片或成骨细胞提前作用，然后再将骨片或成骨细胞分别与破骨细胞共同培养。结果发现当阿仑膦酸钠（10^-11,10^-9,10^-7mol/L)与骨片预处理后，破骨细胞在骨片上形成的骨吸收陷窝数目减少，其抑制率分别为19．5％（P＜0．05）、49．0％（P＜0．01）和74．5％（P＜0．01）。用阿仑膦酸钠（10^-9,10^-7,10^-5mol/L)预处理成骨细胞后，仅在高浓度（10^-5mol/L）时可见破骨细胞骨吸收陷窝的数目明显减少，其抑制率为62．8％（P＜0．01）。结果表明阿仑膦酸钠能直接或通过成骨细胞间接抑制破骨细胞的骨吸收活性。

Effects of Alendronate on rabbit osteoclast function

To explain the potent inhibition mechanisms of Alendronate on the rabbit osteoclasts in vitro by means of bone pit formation assay. Bone silces or osteoblasts were preincubated with various concentrations of Alendronate and then co cultured with osteoclasts. The percent inhibition of pit formation was 19.5%( P <0.05)?49.0%( P <0.01) and 74.5%( P <0.01) respectively when bone slices were preincubated with various concentrations of Alendronate (10 -11 ,10 -9 ,10 -7 mol/L). After preincubation of osteoblasts with various concentrations of Alendronate (10 -9 ,10 -7 ,10 -5 mol/L) Alendronate inhibited the osteoclast mediated bone resportion (62.8% ( P <0.01)) only at the highest concentration (10 -5 mol/L). The results showed that Alendronate inhibited osteoclast activity either directly or indirectly through the osteoblast.