| 分析超速离心法测定生物大分子的沉降系数和分子量 |
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| 引用本文： | 杨渝珍,李东,覃璐.分析超速离心法测定生物大分子的沉降系数和分子量[J].华中科技大学学报(医学版),1987(1). |
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| 作者姓名： | 杨渝珍 李东 覃璐 |
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| 作者单位： | 同济医科大学实验医学研究中心医用分子生物学研究室
(杨渝珍，李东)，同济医科大学实验医学研究中心医用分子生物学研究室(覃璐) |
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| 摘 要： | 离心沉降分析技术是分子生物学基本实验技术之一。3年来,我们用Model-282分析型超速离心机测定了蛋白质、核酸及多糖的分子量(M,沉降-扩散法)和沉降系数(S)80多次,均获得满意结果,为鉴定这些生物大分子的理化特性提供基本数据,本文对该技术原理、方法及测定实例作详细介绍。实验表明,它是测定生物大分子特性的简便、精确、可靠的基准方法。
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| 关 键 词： | 扩散系数 分子量 超速离心沉降分析 沉降系数 |
An Analytical Ultracentrifugation Method for the Measurement of Molecular Weight and Sedimentation Coefficient of Biological Macromolecules |
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| Yang Yuzhen,Li Dong,Qin Lu.An Analytical Ultracentrifugation Method for the Measurement of Molecular Weight and Sedimentation Coefficient of Biological Macromolecules[J].Journal of Huazhong University of Science and Technology(Health Sciences),1987(1). |
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| Authors: | Yang Yuzhen Li Dong Qin Lu |
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| Institution: | Yang Yuzhen,Li Dong,Qin LuDepartment of Medical Molecular Biology,Experimental Medical Research Center,Tongji Medical University,Wuhan |
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| Abstract: | During the past three years, we measured the moleoular weight(M.W) and sedimentation coefficient (S) of some proteins, nucleic acids and polysacchari des up to 80 times, using Hitachi Model-282 analytical ultracentrifuge. In this paper, we introduced principle and method in detail for the determination of sedimentation coefficient by Schlieren and Absorption scanning optical systems and for the determination of molecular weight by the method of combining boundary sedimentation with synthetic boundary procedure. All of the results obtained are satisfactory. Experimental error amounts to 3-5% permitted by this instrument. We expect that the precision of results may be improved by changing our tools used for measurement of the related parameters. |
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| Keywords: | diffusion coefficient molecular weight sedimentation coefficient ultracentrifugal sedimentation analysis |
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