Hepatitis B virus genotype E surface antigen detection with different immunoassays and diagnostic impact of mutations in the preS/S gene |
| |
Authors: | Christophe M Olinger Bernard Weber Jesse A Otegbayo Wim Ammerlaan Natascha van der Taelem-Brulé Claude P Muller |
| |
Affiliation: | Institute of Immunology, National Public Health Laboratory, 20a, rue Auguste Lumière, 1950, Luxembourg, Luxembourg. Christophe.Olinger@LNS.ETAT.LU |
| |
Abstract: | The major neutralizing epitope, the “a” determinant of the hepatitis B virus (HBV) genotype E surface antigen (HBsAg) is most
divergent from that of genotype A, which is used for preparing monoclonal antibodies used in commercially available HBV reagents.
To evaluate the performance of the latest generation of HBsAg detection assays with respect to genotype E HBsAg. Three commercial
assays were evaluated using sera from 200 Nigerian patients compared to the preS/S sequence of DNA positive samples. Out of
200 samples, 61 and 103 gave concordant positive and negative results between the three HBsAg assays. Of 36 samples with discordant
results, 35 were confirmed negative by neutralisation. One of the three assays showed significantly high rate of false positives
(29 of 35). DNA positive samples with no detectable HBsAg or reduced HBsAg detection signals (<75% of mean signal obtained
with HBsAg positive samples) revealed several mutations (V14A, F46S, N48T, L49R, I49T, D51G, A53V, P54L, Q82P, F83C, L127P,
A184V, T189I, S204N, V224A), mostly outside the a-determinant. Several of these mutations are found as wild type nucleotides
normally in genotype A and only exceptionally in genotype E. All three assays showed comparable sensitivities for genotype E
HBsAg detection (98.4–100%) but differed considerably in specificity (84–99%). Failure to detect HBsAg antigen and differences
in signal intensity were mainly associated with mutations in the preS/S gene outside the “a” determinant. |
| |
Keywords: | Genotype E HBsAg Nucleic acid amplification test PreS/S gene |
本文献已被 PubMed SpringerLink 等数据库收录! |
|