| 胞外Ca2+浓度对辐射诱导HL-60细胞凋亡的影响 |
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| 引用本文： | 何子毅,孟庆勇. 胞外Ca2+浓度对辐射诱导HL-60细胞凋亡的影响[J]. 中国辐射卫生, 2005, 14(4): 257-258 |
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| 作者姓名： | 何子毅 孟庆勇 |
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| 作者单位： | 广东医学院分析中心,广东,湛江,524023 |
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| 基金项目： | 广东省卫生厅科学基金项目资助（A2002466） |
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| 摘 要： | 目的探讨胞外不同Ca^2＋浓度对辐射诱导HL-60细胞凋亡的促进作用.方法对体外培养的HL-60细胞经一定剂量的32P辐射后发现加入不同浓度的Ca^2＋继续培养24、48h,流式细胞仪检测凋亡率.结果胞外Ca^2＋作用于HL-60细胞24、48h后发现对辐射诱导细胞凋亡随Ca^2＋浓度的增加细胞凋亡率增大,Ca^2＋有明显的促凋亡作用,相关性很好r24=0.9001（P=0.0145）,r48=0.9343（P=0.0063）.结论胞外不同浓度的Ca^2＋对辐射诱导HL-60细胞有明显的促进凋亡的作用.
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| 关 键 词： | Ca^2＋ 辐射 细胞凋亡 HL-60 |
| 文章编号： | 1004-714X（2005）04-0257-02 |
| 收稿时间： | 2005-04-29 |
| 修稿时间： | 2005-04-29 |
[Ca2+]i in Exterior of Cells Effected on Apoptosis of HL-60 Cells Induced by Irradiation |
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| HE Zi-yi,MENG Qing-yong. [Ca2+]i in Exterior of Cells Effected on Apoptosis of HL-60 Cells Induced by Irradiation[J]. Chinese Journal of Radiological Health, 2005, 14(4): 257-258 |
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| Authors: | HE Zi-yi MENG Qing-yong |
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| Affiliation: | Analysis Center of Guang dong Medical College 524023. |
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| Abstract: | Objective To investigate of the different [Ca~ 2+ ]i in exterior of cells promotion function on apoptosis of HL-60 cells induced by irradiation.Methods To put ration dose ~ 32 P and different [Ca~ 2+ ]i into culture of HL-60 and measure the apoptosis rate with FCM after 24 and 48 hours.Result Apoptosis rate increased with the increase of [Ca~ 2+ ]i which shows an obvious function to promote apoptosis,r_24 =0.9001(P=0.0145);r48=0.9343(P=0.0063).Conclusion [Ca~ 2+ ]i in exterior of cells has a obvious function in promoteing apoptosis induced by irradiation. |
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| Keywords: | Ca~ 2+ Irradiation Cell Apoptosis HL-60 |
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