HLA-DQA1基因多态性与HBV感染结局相关

目的探讨中国汉族人群人类白细胞抗原(HLA)-DQA1基因多态性是否与乙型肝炎病毒(HBV)感染结局相关联。方法以213例HBV自限性感染者和420例慢性乙肝患者为研究对象,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术进行HLA-DQA1基因分型,用EPI和SPSS软件分析DQA1多态性的分布频率及其组间差异。结果DQA1*0102在慢性乙肝组的分布频率显著低于HBV自限性感染组(15.47%比较20.42%,P<0.05),而DQA1*0201在慢性乙肝组的分布频率显著高于HBV自限性感染组(10.48%比较6.10%,P<0.05)。调整性别、年龄等混杂因素影响的非条件logistic回归分析结果显示,与HLA-DQA1其他等位基因相比,携带DQA1*0102者降低慢性乙肝发生的风险(P<0.05,OR=0.69,95%C I:0.49-0.96),而携带DQA1*0201者增加慢性乙肝发生的风险(P<0.05,OR=1.77,95%C I:1.09-2.87)。结论HLA-DQA1基因多态性可能是影响HBV感染结局的重要宿主遗传因素。

Association between HLA-DQA1 gene polymorphism and the outcomes of hepatitis B virus infection

Objective To explore whether human leukocyte antigen(HLA)-DQA1 gene polymorphisms are associated with the outcome of hepatitis B virus(HBV) infection in Chinese Han population.Methods Polymerase chain reaction-sequence specific primer(PCR-SSP) was used to genotype DQA1 gene.213 spontaneously recovered HBV subjects and 420 chronic hepatitis B(HB) patients were recruited.The frequency distributions of genotypes between two groups were analyzed by EPI and SPSS software.Results The frequency of DQA1*0102 allele in chronic HB patients group(patient group) was 15.47%,significantly lower than 20.42% in spontaneously recovered HBV subjects group(subject group)(P<0.05),and the frequency of DQA1*0201 allele in the patients group was 10.48%,significantly higher than 6.10% in the subjects group(P<0.05).The results that were adjusted the confounders with unconditional logistic regression analysis showed a decreased risk of chronic HBassociated with carrying the HLA-DQA1*0102 allele as compared with the other alleles of HLA-DQA1 locus(P<0.05,OR=0.69,95% CI:0.49-0.96),however,an increased risk of chronic HB associated with those carrying DQA1*0201(P<0.05,OR=1.77,95% CI:1.09-2.87).Conclusion HLA-DQA1 gene polymorphisms may play an important role in the outcomes of HBV infection.