| 壳寡糖的制备及组分分析 |
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| 引用本文： | 纪莹, 赵轶, 周翔, 翁登坡, 赵鲁杭. 壳寡糖的制备及组分分析[J]. 中国现代应用药学, 2003, (3): 195-196. |
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| 作者姓名： | 纪莹 赵轶 周翔 翁登坡 赵鲁杭 |
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| 作者单位： | 1. 浙江大学医学院生化教研室,浙江,杭州,310006
2. 浙江大学医学院附属妇产科医院,浙江,杭州,310006 |
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| 基金项目： | 浙江省海洋局资助(9812 10 2 )，浙江省大仪办(0 10 84)资助 |
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| 摘 要： | 目的 研究以壳六糖为主的壳寡糖制备工艺并对制备物组分进行分析。方法 用酶解法水解壳聚糖，有机溶剂法提取壳寡糖，色谱法分离纯化壳六糖，HPLC法分析检测壳寡糖组分。结果 壳寡糖粗提物中的主要成分为6-7聚壳寡糖，色谱分离得到较纯的壳六糖。结论 该制备工艺可以得到以6-7聚壳寡糖为主的壳寡糖制备物。
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| 关 键 词： | 壳寡糖 制备 检测 |
| 文章编号： | 1007-7693(2003)03-0195-02 |
The preparation of chitosan oligomer and the components analysis |
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| JI Ying, ZHAO Yi, ZHOU Xiang, WENG Deng-po, ZHAO Lu-hang. The preparation of chitosan oligomer and the components analysis[J]. Chinese Journal of Modern Applied Pharmacy, 2003, (3): 195-196. |
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| Authors: | JI Ying ZHAO Yi ZHOU Xiang WENG Deng-po ZHAO Lu-hang |
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| Abstract: | OBJECTIVE To study the preparation technology of chitohexose that is the main component, and to analyze the component of the preparing product. METHOD Chitosan was hydrolized with enzyme, then the chitosan oligomer was extrated with organic solvent, following by purifying the chitohexose by chromatograph method and detecting the chitosan oligomer component by HPLC. RESULTS The main component of the crude chitosan oligomer extract was 6 7 oligomer, the chitohexaose could be seperated by chromatograph.CONCLUSION The chitosan oligomer in which the main component was 6 7 oligomer could be produced by preparation technology. |
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| Keywords: | chitosan oligomer preparation detection |
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