| 冬凌草甲素对HepG2细胞内质网应激蛋白IRE-1、PERK和CHOP的作用研究 |
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| 引用本文： | 王辉,叶燕,禹志领.冬凌草甲素对HepG2细胞内质网应激蛋白IRE-1、PERK和CHOP的作用研究[J].中药新药与临床药理,2012,23(3):263-266. |
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| 作者姓名： | 王辉 叶燕 禹志领 |
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| 作者单位： | 1. 广东药学院生命科学与生物制药学院,广州,510006
2. 香港浸会大学中医药学院,香港九龙塘,999077 |
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| 基金项目： | 广东省2011年建设中医药强省科研课题(20111250) |
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| 摘 要： | 目的 研究内质网应激对冬凌草甲素处理的肝癌细胞HepG2的作用.方法 采用特异性小干扰RNA(siRNA)转染HepG2细胞72 h抑制内质网应激蛋白RNA激活蛋白激酶样内质网激酶(PERK)、抑制物阻抗性酯酶1(IRE-1)和CCAAT增强子结合蛋白同源蛋白(CHOP)表达后,MTT法观察40 μmol· L-1冬凌草甲素处理12h的转染细胞的细胞存活率.结果 PERK和IRE-1 siRNA转染抑制相应蛋白表达后增加冬凌草甲素诱导的HepG2细胞死亡(P＜0.05)；而抑制CHOP表达对冬凌草甲素处理的HepG2细胞存活率没有影响(P＞0.05).结论 冬凌草甲素诱导的内质网应激对HepG2细胞具有保护作用.
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| 关 键 词： | 冬凌草甲素 内质网应激 RNA激活蛋白激酶样内质网激酶 抑制物阻抗性酯酶1 CCAAT增强子结合蛋白同源蛋白 |
Role of Endoplasmic Reticulum Stress Proteins IRE-1,PERK and CHOP in Oridonin-treated HepG2 Cells |
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| WANG Hui
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YE Yan
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YU Zhiling.Role of Endoplasmic Reticulum Stress Proteins IRE-1,PERK and CHOP in Oridonin-treated HepG2 Cells[J].Traditional Chinese Drug Research & Clinical Pharmacology,2012,23(3):263-266. |
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| Authors: | WANG Hui YE Yan YU Zhiling |
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| Institution: | 1.School of Life Science and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China;2.School of Chinese Medicine,Hong Kong Baptist University,Kowloon Tong 999077 Hong Kong,China) |
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| Abstract: | Objective To investigate the role of endoplasmic reticulum(ER) stress in oridonin-treated HepG2 cells.Methods We used small interfering RNA(siRNA) for the transfection of HepG2 cells for 72 h to knockdown the expression of ER stress proteins such as RNA-activated protein kinase-like endoplasmic reticulum kinase(PERK),inhibitor resistant esterase 1(IRE-1) and CCAAT/enhancer-binding protein homologous protein(CHOP).The cell viability of transfected HepG2 cells with 40 μmol·L-1 oridonin treatment for 12 h was evaluated by MTT assay.Results Knockdown of PERK or IRE-1 with specific siRNA increased oridonin-induced cell death(P<0.05),while suppression of CHOP expression showed no effect on cell viability(P>0.05).Conclusion Oridonin-induced ER stress shows protective effect on HepG2 cells. |
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| Keywords: | Oridonin Endoplasmic reticulum stress RNA-activated protein kinase-like endoplasmic reticulum kinase Inhibitor resistant esterase 1 CCAAT/enhancer-binding protein homologous protein |
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