自噬相关基因PTEN和Beclinl在乳头状甲状腺癌中的表达和意义

目的检测自噬相关基因PTEN和Beclinl在乳头状甲状腺癌中的蛋白表达,并探讨其与乳头状甲状腺癌发生、发展的相关性及意义。方法应用免疫组化染色法检测78例乳头状甲状腺癌和45例癌旁正常黏膜组织(距离癌组织>2cm)标本自噬相关基因PTEN和Beclinl的蛋白表达,并结合临床病理因素进行分析。结果乳头状甲状腺癌组织中PTEN和Beclinl的阳性表达率分别为41.0%和43.6%,显著低于正常组织的77.8%和73.3%(P<0.05);PTEN与乳头状甲状腺癌的包膜侵犯程度、淋巴结转移、性别、年龄无关(P>0.05);Beclinl与乳头状甲状腺癌的淋巴结转移有关(P<0.05),与包膜侵犯程度、性别、年龄无关(P>0.05)。PTEN与Beclinl在乳头状甲状腺癌中的表达呈正相关(r=0.532)。结论自噬相关基因PTEN和Beclinl在乳头状甲状腺癌组织中蛋白表达下调,并与肿瘤淋巴结转移有关。自噬活性的改变可能与乳头状甲状腺癌的发生、发展相关。

Relationship of autophagy-related gene PTEN and Beclinl in papillary thyroid carcinoma

Objective To detect the protein expression of autophagy-related gene PTEN and Beclinl in papillary thyroid carcinoma,and discuss the correlation and significance of its with occurrence,development of papillary thyroid carcinoma.Methods Using immunohistochemical staining method to detect papillary thyroid carcinoma 78 cases of thyroid carcinoma,45 cases of cancer by normal mucosa tissue（distance cancerous tissue2cm） autophagy Beclinl gene related PTEN and the protein expression,and combined with clinical pathological factors were analyzed.Results Papillary thyroid carcinoma tissues PTEN and Beclinl positive expression rate（41.0% and 43.6%） significantly lower than that in normal tissue（77.8% and 73.3%）（P0.05）;PTEN with papillary thyroid carcinoma with the capsule infringe degree,lymph node metastasis,gender,regardless of age had no relationship（P0.05）.Beclinl with thyroid carcinoma of the lymph node metastasis relevant had some relationship（P0.05）,and with coated infringe degree,gender,regardless of age had no relationship（P0.05）.And PTEN with Beclinl in papillary thyroid carcinoma had positively correlated（r=0.532）.Conclusion Autophagy-related gene PTEN and Beclinl in papillary thyroid carcinoma decreased protein expression,and with tumor lymph node metastasis are concerned.Autophagy activity with the change may be related to occurrence and development of papillary thyroid carcinoma.