窦房结区甲醛湿敷法建立犬窦房结损伤模型

目的 探讨甲醛湿敷法建立犬窦房结损伤模犁的可行性和电生理特征变化.方法 20只实验犬按随机数字表法随机分为湿敷后2 h、4 h、1周及4周组(n=5).用20%甲醛湿敷窦房结区建立窦房结损伤模型.各组于湿敷前、湿敷后各自相对应时间点行电生理功能检测,同步记录体表心电图(electrocardiogram,ECG).结果 20只实验犬甲醛湿敷时间为3～12(6.2±2.6)min.5只犬甲醛湿敷后窦性心率(heart rate,HR)显著减慢(140±11)、(89±6)次/min,P<0.01],无心律失常;4只犬除窦性心率明显减慢外出现窦性心律不齐、窦性停搏及频发房性早搏,但很快恢复为规整的窦性节律;11只犬予甲醛湿敷后P波消失,呈交界性逸搏心律,其中4只于湿敷后24 h行ECG监测时转为窦性心律,但HR仍显著低于湿敷前.湿敷后2 h、24 h、1周及4周组的HR均较甲醛湿敷前显著减慢(P<0.01);各组湿敷后的窦房结恢复时间(sinus node recovery time,SNRT)及校正的窦房结恢复时间(corrected sinus node recovery time,CSNRT)则均较湿敷前显著延长(P<0.01,P<0.05).结论 采用甲醛湿敷法可以成功建立犬窦房结损伤模型,其电生理改变与临床病态窦房结综合征表现相似.

Establishment of sinoatrial node damage model in canine induced by formaldehyde wet dressing of sinoatrial node aera

Objective To explore the feasibility and reliability to build a sinoatrial node damage model in canine induced by formaldehyde wet dressing.Methods Twenty dogs were randomly divided into 4 groups(5 in each group) by means of random number table:2-hour,24-hour,1-week and 4-week groups after wet dressing.The sinoatrial node area of canine was damaged by wet dressing with 20% formaldehyde.Sinus node function was measured before,2 h after wet dressing and corresponding times of each group.Electrocardiogram(ECG)...