寨卡病毒感染人神经母细胞瘤细胞所诱导的细胞因子表达水平变化

目的 研究寨卡病毒（ZIKV）感染人神经母细胞瘤细胞（SH-SY5Y）所导致的细胞因子分泌的变化,探讨ZIKV影响神经系统功能紊乱的机制。方法 建立ZIKV感染SH-SY5Y细胞模型,采用噻唑蓝（MTT）分析法检测细胞活力,采用悬浮芯片系统（Bio-Plex^® 200）Luminex多重检测技术同时定量检测24、48、72 h细胞培养上清液中细胞因子IL-1β、IL-2、IL-4、IL-5、IL-13、IL-18、IFN-γ、TNF-α、IL-12P70、GM-CSF的浓度。结果 病毒感染组24、48、72 h细胞存活率均低于正常对照组（均P<0.01）。病毒感染组（24~72 h）分泌IL-1β、IL-5、IFN-γ、TNF-α、IL-12P70、GM-CSF的浓度水平均高于对照组（均P<0.01）。病毒感染组（48 h）分泌的IL-1β、IL-12P70、GM-CSF浓度水平均高于对照组（48 h）,病毒感染组（72 h）分泌TNF-α高于对照组（72h）（P<0.05或P<0.01）。结论 ZIKV感染SH-SY5Y细胞后致其细胞存活率降低,并以时间依赖的方式分泌浓度水平相对较高的促炎性细胞因子。

Changes of cytokine expression levels in human neuroblastoma cells infected with Zika virus

Objective To observe changes in cytokine secretion induced by Zika virus （ZIKV） infection in human neuroblastoma SH-SY5Y and explore the mechanism of ZIKV affecting nervous system dysfunction. Methods A model of SH-SY5Y cells infected with ZIKV was established and the cell viability was detected by MTT assay. Cell culture supernatants were harvested at 24, 48, and 72 hours. Concentrations of IL-1β, IL-2, IL-4, IL-5, IL-13, IL-18, IFN-γ, TNF-α, IL-12P70, and GM-CSF were measured simultaneously and quantitatively using the Bio-Plex^? 200 system. Results The cell survival rates at 24, 48, and 72 hours in the virus infection group were lower than those in the normal control group （all for P <0.01）. The levels of IL-1β, IL-5, IFN-γ, TNF-α, IL-12P70, and GM-CSF secreted by the virus infection group （24-72 h） were higher than those of the control group （all for P <0.01）. The levels of IL-1β, IL-12P70, and GM-CSF secreted by the virus infection group （48 h） were higher than those of the control group （48 h）, and the level of TNF-α secreted by the virus infection group （72 h） was higher than that of the control group （72 h） （P <0.05 or P <0.01）. Conclusion SH-SY5Y cells infected with ZIKV resulted in the decrease in cell viability and the secretion of relatively high concentration of pro-inflammatory cytokines in a time-dependent manner.