鼻咽癌组织中CD44V6 mRNA及其蛋白表达的临床意义

目的：探讨鼻咽癌（NPC）组织中CD44V6 mRNA及其蛋白表达的临床意义。方法：应用催化信号放大原位杂交和免疫组织化学方法，检测65例NPC组织中CD44V6mRNA及其蛋白的表达情况，结合临床病理因素及随访资料进行分析。结果：在NPC组织中CD44V6 mRNA及其蛋白表达阳性率分别为47．7％（31／65）和52．3％（34／65），CD44V6mRNA及其蛋白阳性表达与NPC原发病灶的分期和淋巴结转移均正相关，CD44V6mRNA及其蛋白阳性表达者5年内复发转移率高（P＜0．01），5年以上生存率低（P＜0．05），结论：CD44V6基因表达可作为预测NPC转移，复发和评估预后的重要生物学指标。

The Clinical Significance of the CD44V6 mRNA and Protein Expression in Nasopharygeal Calcinomas

Objective To investigate the expression and clinic significance of CD44V6 mRNA and protein in nasopharygeal carcinoma(NPC).Methods Catalyzed signal amplification(CSA)in situ hybridization and immunohistochemistry were used to detect the expression of CD44V6 mRNA and protein in 65 cases of nasopharygeal carcinoma.The results, combined with clinical pathology index and follow up survey of NPC cases were analysed.Results In NPC tissues, the positive rate of CD44V6 mRNA and protein were 47.7%(31/65) and 52.3%(34/65),respectively.The positive expression of CD44V6 mRNA and protein were positively correlated with the staging of primary NPC and its lymph node metastasis, the patient with positive expression of CD46V6 mRNA and protein had a high recurrence rate and metastasis rate( P <0.01) and a low 5 years survival rate( P <0.05).Conclusion CD44V6 gene could be important pathological biology index to predict the metastasis?recurrence and prognosis of NPC.