| 哮喘小鼠模型中气道重构的变化及地塞米松的干预作用 |
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| 引用本文： | 王敏,李蓓,张光环.哮喘小鼠模型中气道重构的变化及地塞米松的干预作用[J].临床肺科杂志,2010,15(2):184-185. |
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| 作者姓名： | 王敏 李蓓 张光环 |
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| 作者单位： | 宜昌,三峡大学第一临床医学院,宜昌市中心人民医院,湖北,443003 |
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| 摘 要： | 目的探讨哮喘小鼠模型中气道重构的变化及地塞米松的干预作用。方法将小鼠随机分成正常对照组、哮喘模型组、地塞米松治疗组(DXM组),每组10只。哮喘组和DXM组给予卵蛋白(OVA)致敏并反复雾化吸入OVA 2周、4周,DXM组腹腔注射DXM。各组分别于末次雾化激发后进行取材,收集肺组织,制作石蜡切片,HE染色观察气道中嗜酸性粒细胞;测定单位气道面积基底膜周径(Pbm)、管壁总面积(WAt)、内壁面积(WA i)、平滑肌面积(WAm);用免疫组织化学方法检测肺组织中CTGF表达水平。采用SPSS 13.0软件进行统计分析。结果哮喘模型组WAt/Pbm、WA i/Pbm、WAm/Pbm及CTGF表达显著高于对照组,给予DXM可显著缓解上述指标的升高。结论气道重构在哮喘发病早期即已出现,并随着病程的延长而加重,使用地塞米松可部分抑制气道重构。
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| 关 键 词： | 哮喘 小鼠 气道重构 地塞米松 结缔组织生长因子 |
The effect of dexamethasone on airway remodeling in asthmatic mouse |
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| WANG Min,LI Bei,ZHANG Guang-huan.The effect of dexamethasone on airway remodeling in asthmatic mouse[J].Journal of Clinical Pulmonary Medicine,2010,15(2):184-185. |
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| Authors: | WANG Min LI Bei ZHANG Guang-huan |
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| Institution: | .(The First College of Clinical Medical Science, China ; Three Gorges University, Yichang Central People＇s Hospital, Yichang 443003, China) |
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| Abstract: | Objective To explore the effect of dexamethasone(DXM)on airway remodeling in asthmatic mouse.Methods The mice were randomly divided into 3 groups: control group,asthmatic group,and DXM treated group,with 10 mice in each group.The asthmatic group,and the DXM treated group were sensitized and repeatedly challenged with ovalbumin(OVA)for 2 and 4 weeks.The DXM treated group were intraperitoneal injected DXM.After the modle succeded,the mice were lavaged.The eosinophil around the airway was observed after the hi... |
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| Keywords: | dexamethasone asthma mouse airway remodeling connective tissue growth factor |
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