去甲二氢愈创木酸对人恶性胶质瘤细胞系CHG-5生长的影响

目的观察去甲二氢愈创木酸(nordihydroguaiaretic acid,NDGA)对人恶性胶质瘤细胞系CHG-5体内外生长的影响.方法采用MTT法、流式细胞仪、光镜等技术观察CHG-5细胞体外增殖、细胞周期、凋亡和裸鼠移植瘤生长情况.结果NDGA可显著抑制CHG-5细胞体外增殖,G0/G1期细胞明显增多,S、G2/M期细胞减少,凋亡细胞增加.采用接种后第5天按50 mg/kg腹腔内注射给药,治疗组移植瘤体积较对照组缩小,未见明显的毒副作用.结论NDGA对CHG-5细胞的体内外生长具有一定的抑制作用,其作用可能与细胞周期阻滞和诱导细胞凋亡有关.

Effects of NDGA on the growth of a human malignant glioma cell line CHG-5

Objective To investigate the effects of nordihydroguaiaretic acid (NDGA) on the growth of a human malignant glioma cell line CHG 5 in vitro and in vivo . Methods Colorimetric MTT assay, flow cytometry, and light microscopy were used to investigate the proliferation in vitro , cell cycles, apoptosis of CHG 5 cells, and the growth of xenografted tumor in nude mice. Results NDGA significantly inhibited the proliferation of CHG 5 cells in vitro . Cells in G 0/G 1 phase increased, but cells in S, G 2/M phases decreased, and apoptotic cells increased significantly. After treatment of NDGA (50 mg/kg, intraperitoneally) at 5 d after the inoculation of tumor cells, the xenografted tumor volume reduced remarkably without causing significant toxic and side effects. Conclusion The inhibitory effect of NDGA on the growth of CHG 5 cells may be correlated with the regulation of cell cycles and induction of apoptosis.