白眉蝮蛇去整合素rAdinbitor对人肝癌细胞系SMMC-7721细胞黏附与增殖的抑制作用

Objective:To investigate the effects of rAdinbitor on the adhesion and proliferation of human hepatoma cell strain SMMC-7721.Methods:Cell adhesion assay was used to observe the effect of rAdinbitor on the adhesion of SMMC-7721 cells to fibronectin (FN).Crystal violet staining was performed to detect the influence of rAdinbitor on the adhesion of SMMC7721 cells.MTT assay was employed to detect the inhibitory effects of different concentration of rAdinbitor on the proliferation of SMMC-7721 cells.The morphologic changes of the control SMMC-7721 cells and the apoptotic cells induced by 200 μg/mL rAdinbitor for 36 h were observed under light microscope after HE staining.Flow cytometry analysis was applied to determine the apoptosis rate of SMMC-7721 cells.Results:(1) FN promoted the adhesion of human hepatoma cell strain SMMC-7721 in a dose-dependent manner.(2) rAdinbitor could dose-dependently inhibit the adhesion of SMMC-7721 cells to FN.The higher the concentration was,the stronger the inhibition was.There was significant difference among the groups (P＜0.05).(3) rAdinbitor had a strong inhibition on the proliferation of SMMC-7721 cells and showed a dose-dependent manner (P＜0.05).After a 48 h exposure,the IC5o value of rAdinbitor was 177.83 μg/mL.(4) After exposure of SMMC-7721 cells to 200 μg/mL rAdinbitor for 36 h,the eady morphologic changes appeared and the apoptosis rate was 20.68%,significantly higher than that of the control group (2.38%,P＜0.05).Conclusion:rAdinbitor can dose-dependently inhibit the SMMC-7721 cells adhesion to FN,and can inhibit the proliferation in dose-dependent manner and promote their apoptosis.

rAdinbitor,a novel disintegrin from Agkistrodon halys brevicaudus stejneger inhibits adhesion and proliferation of SMMC-7721 cells

Objective  To investigate the effects of rAdinbitor on the adhesion and proliferation of human hepatoma cell strain SMMC-7721. Methods  Cell adhesion assay was used to observe the effect of rAdinbitor on the adhesion of SMMC-7721 cells to fibronectin (FN). Crystal violet staining was performed to detect the influence of rAdinbitor on the adhesion of SMMC-7721 cells. MTT assay was employed to detect the inhibitory effects of different concentration of rAdinbitor on the proliferation of SMMC-7721 cells. The morphologic changes of the control SMMC-7721 cells and the apoptotic cells induced by 200 μg/mL rAdinbitor for 36 h were observed under light microscope after HE staining. Flow cytometry analysis was applied to determine the apoptosis rate of SMMC-7721 cells. Results  (1) FN promoted the adhesion of human hepatoma cell strain SMMC-7721 in a dose-dependent manner. (2) rAdinbitor could dose-dependently inhibit the adhesion of SMMC-7721 cells to FN. The higher the concentration was, the stronger the inhibition was. There was significant difference among the groups (P < 0.05). (3) rAdinbitor had a strong inhibition on the proliferation of SMMC-7721 cells and showed a dose-dependent manner (P < 0.05). After a 48 h exposure, the IC₅₀ value of rAdinbitor was 177.83 μg/mL. (4) After exposure of SMMC-7721 cells to 200 μg/mL rAdinbitor for 36 h, the early morphologic changes appeared and the apoptosis rate was 20.68%, significantly higher than that of the control group (2.38%, P < 0.05). Conclusion  rAdinbitor can dose-dependently inhibit the SMMC-7721 cells adhesion to FN, and can inhibit the proliferation in dose-dependent manner and promote their apoptosis.