猪轮状病毒血凝特性的测定

本文报告了从江苏省天然腹泻仔猪粪便中分离获得并适应于MA-104细胞培养的4株猪轮状病毒的血凝特性,找出了它们的最佳血凝条件及血凝谱。并以牛轮状病毒株(NCDV)进行比较。结果4株猪轮状病毒的最佳血凝条件是用pH7.2～7.4内含0.25％牛血清白蛋白的0.01M的PBS液稀释新鲜红细胞,新鲜红细胞的浓度以0.5％为适宜,温度37℃或26℃。在这最佳的条件下,4株猪轮状病毒的细胞培养毒,对人及多种动物的红细胞产生凝集作用,其中以豚鼠、马、人O型、绵羊、犊牛红细胞凝集最好,其次为人的AB型,山羊及犬的红细胞;而对鸡、小白鼠、兔的红细胞不凝集,对猪的红细胞凝集不稳定。

The Hemagglutinating Characteristics of Four Porcine Rotavirus

Four porcine Rotavirus cell culture strains originally isolated from natural diarrheal piglet fecal samples from Jiangsu province were used to study the hemagglutinating characteristics.It revealed that all the four strains have the optimal agglutination to human and several kinds of domestic animal RBC,especially are more efficient to guinea pig,horse,human group O,calf and sheep,under the condition of the standard 0.5% RBC suspension diluted in 0.1MPBS,supplemented with 0.25% bovine serum albumin at pH 7.2-7.4,at 37℃ or 26℃.In addition,it also demonstrated that there is partial agglutination in human group AB,goat and dog RBC,but not in chic-ken,mice,and rabbit at the above-mentioned condition.