肾细胞癌基质金属蛋白酶-9与血管生成的关系

目的观察基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在肾细胞癌组织中的表达特点及其与微血管密度(microvessel density,MVD)的关系,探讨MMP-9对肾细胞癌血管生成的作用和意义.方法采用SP免疫组织化学染色方法检测55例肾细胞癌组织中MMP-9和CD34的表达,并结合临床病理参数进行综合分析.结果 MMP-9蛋白在肾细胞癌组织中的阳性率为63.63%.肾癌组织中MMP-9的表达显著高于正常肾组织(P＜0.05),且与肿瘤中MVD密切正相关(r=0.456,P＜0.01).MMP-9的表达和肿瘤中MVD均与肾癌的分期、肾包膜侵袭和淋巴结转移密切相关(P＜0.05),而与肾癌组织学类型无关(P＞0.05).结论肾癌组织MMP-9高表达与MVD及肾癌的一些生物学行为、转移发生有密切关系,MMP-9可能是肾癌的重要促血管生成因子之一.

Relationship between MMP-9 and angiogenesis in renal cell cacinoma

Objective To explore the role and the significance of matrix metalloproteinase-9 (MMP-9) on angiogenesis in renal cell carcinoma (RCC) through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in RCC. Methods The expression of MMP-9 and CD34 in RCCs specimens were detected by immunohistochemical technique, and the clinical pathological pa- rameters were analyzed at the same time. Results The positive rate of MMP-9 was 63.63% in RCC tissue. The expression of MMP-9 in RCC tissue, being significantly correlated with MVD in tumor (r= 0.456, P<0.01), was remarkably higher than that in normal renal tissue ( P < 0.05). MMP-9 expression and MVD in tumor were closely related ID clinical stage, renal fibromembrane invasion and lymph node metastasis (P<0.05), but not related to pathological types of RCC (P>0.05). The higher the tumor stage, the higher the expression of MMP-9 and MVD, MMP-9 and MVD's expression were significantly higher in invasion and metastasis than in no invasion and metastasis. Conclusion The high expression of MMP-9 was significantly associated with MVD, biological behaviors of RCC, MMP-9 might be one of the important factors in the angiogenesis of RCC.