| 蓖麻毒素及其A、B链的提取分离纯化的研究 |
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| 引用本文： | 许金熀,王雄莉,任建人,鲁纯素,谢蜀生,马大龙,龙振洲.蓖麻毒素及其A、B链的提取分离纯化的研究[J].北京大学学报(医学版),1989(2). |
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| 作者姓名： | 许金熀 王雄莉 任建人 鲁纯素 谢蜀生 马大龙 龙振洲 |
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| 作者单位： | 北京医科大学物理化学教研室
(许金熀，王雄莉，任建人，鲁纯素)，北京医科大学免疫学教研室
(谢蜀生，马大龙)，北京医科大学免疫学教研室(龙振洲) |
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| 摘 要： | 本文研究蓖麻毒素及其A、B链的提取、分离、纯化,根据B链能与Sepharose-6 B上的半乳糖结合,而A链不具有这一性质,用β-巯基乙醇使蓖麻毒素直接在Sepharose-6B柱上断链,然后分别用Tris-HCl和Tris-HCl-半乳糖溶液洗脱,首次把蓖麻毒素的制备、纯化以及A、B链的断链、分离结合起来,大大简化了工艺。
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| 关 键 词： | 蓖麻毒素 蓖麻毒素A链 蓖麻毒素B链 |
PURIFICATION OF RICIN, RICIN A AND B CHAINS |
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| Institution: | Department of Physical Chemistry Xu Jinhuang,et al |
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| Abstract: | This paper describes a method for the preparation of ricin and its constituent peptide chains, namely ricin A and B chains. The purification involved extraction from castor beans by using 5% acetic acid solution and chromatography on a CM52 column and subsequently on a sepharose-6B column. The sepharose-6B column was first washed with 10 mmol/L Tris-HC1 (pH=7.7), then the intact ricin was split into A and B chains on the sepharose-6B column with 2-mercaptoethanol. The A chain was eluted with Tris-HCl buffer containing 2-mercaptoethanol. The B chain was eluted with Tris-HCl buffer containing galactose. The B chain was further purified by chromatography on a DEAE-csllulose column. The A chain was further purified by chromatography on a DEAE-cellulose column and a CM 52 column. |
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| Keywords: | Ricin Ricin A chain Ricin B chain |
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