外用细菌纤维素影响兔耳创面愈合的形态学及组织学观察

目的：观察细菌纤维素（BC）对创面愈合的影响。方法：应用新西兰大耳白兔18只,随机分成空白对照（A组）、BC（1∶5）实验（B组）、BC（1∶6）实验（C组）、BC（1∶8）实验（D组）、纳米银对照（E组）、油纱对照（F组）,建立兔耳皮肤创面愈合模型,通过不同时间点观察创面愈合的形态学及组织学变化。结果：①形态学观察：实验（B、C、D）组能够有效促进兔耳创面愈合,对照组间疗效并无显著区别;②HE染色：创面愈合期（0～14天）同一时间点,实验组的毛细血管和肉芽组织生长较对照组丰富;③Masson三色法胶原染色：对照（A、E、F）组创面的胶原纤维稍粗大,排列稍紊乱;B、C、D实验组创面的胶原纤维较细,排列较整齐;④免疫组化染色：α-SMA特异性阳性表达的程度为：D组〉B组〉C组〉A组〉E组〉F组。结论：BC能有效促进创面愈合,减轻瘢痕形成,且细菌纤维素（1∶8）组是促进创面愈合的最适敷料治疗组。

Morphology and histological observation of external use of bacterial cellulose influencing on rabbit ear would healing

0bjective To observe the influences of Bacterial Cellulose（BC） on would healing.Methods Using 18 New Zealand rabbits with big ears,which were randomly divided into the blank control group（Group A）,BC（1：5） experimental group（Group B）,BC（1：6） experimental group（Group C）,BC（1：8） experimental group（Group D）,Nano-silver control group（Group E）,MEBO gauze control-group（Group F）.Establishing the rabbits ears skin would healing model by observing the would healing morphology and histological variation on different periods.Results ① Morphology observig： Experiment groups（B、C、D） could improve the wound healing of rabbits ear.There were no distinguished difference effects on control group.② HE Staining：would healing period（Days 0-14） at same period,the growth of capillaries and granulation tissue was more rich in experimental group compared with contol-group.③Masson＇s trichrome staining of collagen： The collagen fiber of control group（A、E、F） was more thickening and derangement.The collagen fiber of experimental group（B、C、D） was thinner and arranged more in order.④ The immunohistochemical staining： The degree of positive expression of α-SMA were：Group D〉Group B〉Group C〉Group A〉Group E〉Group F.Conclusion BC could promoting the process of wound healing efficiencily,and reducing the formation of scar.Also BC（1：8） group was the most suitable for would dressing group of improving would healing.